ArticleBMC pharmacology & toxicology2020
Isoniazid causes heart looping disorder in zebrafish embryos by the induction of oxidative stress.
Article in BMC pharmacology & toxicology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 18 citations in OpenAlex.
- Incorporation of a Natural Deep Eutectic Solvent-Based System as a Cryoprotectant in Solid Lipid Nanoparticles: Advancing toward Industrial Scalability.ACS nanoscience Au · 2025Article
- The Effects of Kynurenic Acid in Zebrafish Embryos and Adult Rainbow Trout.Biomolecules · 2024Article
- Cardiac Tuberculosis: A Case Series from Ethiopia, Italy, and Uganda and a Literature Review.The American journal of tropical medicine and hygiene · 2024Review
- Loss ofFrontiers in neuroscience · 2023Article
- Impacts of Cetylpyridinium Chloride on the Survival, Development, Behavior, and Oxidative Stress of Early-Life-Stage Zebrafish (Antioxidants (Basel, Switzerland) · 2022Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cardiotoxicity of isoniazid on zebrafish embryos and its underlying mechanism is unclear.
methodsHere, we exposed zebrafish embryos at 4 h post-fertilization to different levels of isoniazid and recorded the morphology and number of malformed and dead embryos under the microscope.
resultsThe high concentration of isoniazid group showed more malformed and dead embryos than the low concentration of isoniazid group and control group. The morphology of the heart and its alteration were visualized using transgenic zebrafish (cmlc2: GFP) and confirmed by in situ hybridization. The negative effects of isoniazid on the developing heart were characterized by lower heart rate and more heart looping disorders. Mechanistically, PCR showed decreased expression of heart-specific transcription factors when exposed to isoniazid. Oxidative stress was induced by isoniazid in cardiomyocytes, mediated by decreased activities of catalase and superoxide dismutase, which were rescued by scavengers of reactive oxygen species.
conclusionIn conclusion, this study demonstrated that isoniazid led to heart looping disturbance by the downregulation of cardiac-specific transcription factors and induction of cardiomyocyte apoptosis.
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