ArticlePloS one2020
Towards low cost, multiplex clinical genotyping: 4-fluorescent Kompetitive Allele-Specific PCR and its application on pharmacogenetics.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Development and application of a cost-effective multiplex Kompetitive Allele-Specific polymerase chain reaction assay for pyramiding resistant genes of fusarium head blight and powdery mildew in wheat.BMC plant biology · 2025Article
- Determining Zygosity With Multiplex Kompetitive Allele-Specific PCR (mxKASP) Genotyping.Ecology and evolution · 2025Article
- Pharmacogenetic variations and clinical implications of actionable CYP2D6/CYP2C19 variants in Central Indian patients with common mental disorders.Frontiers in pharmacology · 2025Article
- Genetic polymorphism in European and African sheep breeds reared in Hungary based on 48 SNPs associated with resistance to gastrointestinal parasite infection using KASP-PCR technique.Tropical animal health and production · 2023Article
- Molecular Genetic Techniques in Biomarker Analysis Relevant for Drugs Centrally Approved in Europe.Molecular diagnosis & therapy · 2022Review
- K-seq, an affordable, reliable, and open Klenow NGS-based genotyping technology.Plant methods · 2021Article
- KASP: a genotyping method to rapid identification of resistance in Plasmodium falciparum.Malaria journal · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
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Abstract
Single-nucleotide polymorphisms (SNPs) is associated with efficacy of specific drugs. Although there are several methods for SNP genotyping in clinical settings, alternative methods with lower cost, higher throughout and less complexity are still needed. In this study, we modified Kompetitive Allele Specific PCR to enable multiplex SNP genotyping by introducing additional fluorescent cassettes that specifically help to differentiate more amplification signals in a single reaction. This new format of assay achieved a limit of detection down to 310 copies/ reactions for simultaneous detection of 2 SNPs with only standard end-point PCR workflow for synthetic controls, and genotyped 117 clinical samples with results that were in 100% agreement with hospital reports. This study presented a simplified, cost-effective high-throughput SNP genotyping alternative for pharmacogenetic variants, and enabled easier access to pharmaceutical guidance when needed.
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