ArticleJournal of visualized experiments : JoVE2020
LarvaSPA, A Method for Mounting Drosophila Larva for Long-Term Time-Lapse Imaging.
Article in Journal of visualized experiments : JoVE, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- Protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes.STAR protocols · 2026Article
- Immobilizing Second-InstarCold Spring Harbor protocols · 2025Article
- Endocytosis of Wnt ligands from surrounding epithelial cells positions microtubule nucleation sites at dendrite branch points.PLoS biology · 2025Article
- In vivo optogenetic manipulations of endogenous proteins reveal spatiotemporal roles of microtubule and kinesin in dendrite patterning.Science advances · 2024Article
- Protocol for Imaging the Same Class IV Neurons at Different Stages of Development.Bio-protocol · 2024Article
- Light-induced trapping of endogenous proteins reveals spatiotemporal roles of microtubule and kinesin-1 in dendrite patterning ofbioRxiv : the preprint server for biology · 2023Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2023Article
- A photo-switchable assay system for dendrite degeneration and repair inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Phagocytosis and self-destruction break down dendrites ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector.Journal of visualized experiments : JoVE · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Live imaging is a valuable approach for investigating cell biology questions. The Drosophila larva is particularly suited for in vivo live imaging because the larval body wall and most internal organs are transparent. However, continuous live imaging of intact Drosophila larvae for longer than 30 min has been challenging because it is difficult to noninvasively immobilizeimmobilizing larvae for a long time. Here we present a larval mounting method called LarvaSPA that allows for continuous imaging of live Drosophila larvae with high temporal and spatial resolution for longer than 10 hours. This method involves partially attaching larvae to the coverslip using a UV-reactive glue and additionally restraining larval movement using a polydimethylsiloxane (PDMS) block. This method is compatible with larvae at developmental stages from second instar to wandering third instar. We demonstrate applications of this method in studying dynamic processes of Drosophila somatosensory neurons, including dendrite growth and injury-induced dendrite degeneration. This method can also be applied to study many other cellular processes that happen near the larval body wall.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.