ArticleAnnals of translational medicine2020
Single-cell transcriptomics reveals the alteration of peripheral blood mononuclear cells driven by sepsis.
Article in Annals of translational medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Single-cell transcriptomics reveals pathogen-specific monocyte heterogeneity and potential biomarkers in gram-positive versus gram-negative bloodstream infections.World journal of emergency medicine · 2026Article
- Single-cell multi-omic landscape reveals anatomical-specific immune features in adult and pediatric sepsis.Nature immunology · 2026Article
- Impaired peripheral mononuclear cell metabolism in patients at risk of developing sepsis: A cohort study.Journal of critical care medicine (Universitatea de Medicina si Farmacie din Targu-Mures) · 2026Article
- Single-cell RNA transcriptomics divulged altered gene expressions in monocytes and HLA variations in decompensated cirrhosis patients with sepsis.Scientific reports · 2025Article
- Emerging Role of Long, Non-Coding RNA Nuclear-Enriched Abundant Transcript 1 in Stress- and Immune-Related Diseases.International journal of molecular sciences · 2025Review
- ITGB2 and ICAM3 predict increased survival of sepsis with decreased intercellular communication in cytotoxic CD8+ T cells.Scientific reports · 2025Article
- Single-Cell Landscape of Bronchoalveolar Lavage Fluid Identifies Specific Neutrophils during Septic Immunosuppression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Assessing the Influence of Selected Permeabilization Methods on Lymphocyte Single-Cell Multi-Omics.Antibodies (Basel, Switzerland) · 2025Article
- Multi-omic analysis of PBMCs in sepsis reveals widespread cytotoxic dysfunction and an increased population ofFrontiers in immunology · 2025Article
- Comprehensive analysis of the role of diverse programmed cell death patterns in sepsis.Frontiers in immunology · 2025Article
- Portfolio analysis of single-cell RNA-sequencing and transcriptomic data unravels immune cells and telomere-related biomarkers in sepsis.Frontiers in immunology · 2025Article
- Deciphering the molecular and cellular atlas of immune cells in septic patients with different bacterial infections.Journal of translational medicine · 2023Review
- Single-cell RNA sequencing deciphers the mechanism of sepsis-induced liver injury and the therapeutic effects of artesunate.Acta pharmacologica Sinica · 2023Article
- Single-cell transcriptome analysis reveals the regulatory effects of artesunate on splenic immune cells in polymicrobial sepsis.Journal of pharmaceutical analysis · 2023Article
- The tempo and mode of gene regulatory programs during bacterial infection.Cell reports · 2022Article
- Single-cell transcriptome profiling of the immune space-time landscape reveals dendritic cell regulatory program in polymicrobial sepsis.Theranostics · 2022Article
- Novel Circulating and Tissue Monocytes as Well as Macrophages in Pancreatitis and Recovery.Gastroenterology · 2021Article
- Sepsis: deriving biological meaning and clinical applications from high-dimensional data.Intensive care medicine experimental · 2021Review
- Article
- The Diverse Roles of Monocytes in Cryptococcosis.Journal of fungi (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSepsis is a serious systemic inflammatory response syndrome caused by infection, with an extremely high mortality rate. Peripheral blood mononuclear cells (PBMCs) played a key role in the immune response against infection, whose components and functions were altered radically in Sepsis. Here, we wondered to characterize the alteration of PBMCs in sepsis at the single-cell transcriptional level.
methodsWe isolated PBMCs from seven septic patients and four donors. Based on BD Rhapsody, PBMCs were generated by single-cell RNA sequencing, and cell types were clustered and named by unsupervised clustering and annotation analysis.
resultsPBMCs were profiled for 6 kinds of cell types, the biological properties of T cell and monocytes were shown in a detailed manner. We noticed that monocytes could be clustered into 6 subsets, with great heterogeneity in the alteration of composition, gene profile, and signaling pathways driven by sepsis. Moreover, the expression of representative genes was high associated with septic clinical indicators in clusters of monocytes, such as NEAT1.
conclusionsAlthough the study was preliminary, we revealed sepsis-specific alteration of PBMCs and associated pathways. These results give a panoramic picture of PBMCs in composition, genes profiles, and pathway signatures that are driven by sepsis, which offers a unique perspective to understand disease progression or treatment in clinical practice.
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