Evidence map›Paper›PMID 32174900›Full record

ArticleFrontiers in microbiology2020

Biophysical Characterization of Membrane Phase Transition Profiles for the Discrimination of Outer Membrane Vesicles (OMVs) From

Angelo Sarra, Antonella Celluzzi, Stefania Paola Bruno, Caterina Ricci, Simona Sennato, Maria Grazia Ortore, Stefano Casciardi, Federica Del Chierico, Paolo Postorino, Federico Bordi and 1 more

Open access · greenAbstract read
In one paragraph

Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 35 citations in OpenAlex.

  1. Article
  2. Review
  3. Optimizing genetic engineering approaches for protein loading into bacterial extracellular vesicles for vaginal drug delivery.Journal of controlled release : official journal of the Controlled Release Society · 2026
    Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Review
  9. Outer Membrane Vesicles Formed by ClinicalMolecules (Basel, Switzerland) · 2024
    Article
  10. Article
  11. Article
  12. Review
  13. Review
  14. Tween-20 Induces the Structural Remodeling of Single Lipid Vesicles.The journal of physical chemistry letters · 2022
    Article
  15. Article
  16. Article
  17. Extracellular Vesicles Derived FromFrontiers in nutrition · 2021
    Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 6 institutions in 1 country.

Angelo SarraDepartment of Science, University of Roma Tre, Rome, Italy.
Antonella CelluzziResearch Laboratories, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Stefania Paola BrunoResearch Laboratories, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Caterina RicciDepartment of Life and Environmental Sciences, Marche Polytechnic University, Ancona, Italy.
Simona SennatoCNR-ISC UOS Sapienza and Department of Physics, Sapienza University of Rome, Rome, Italy.
Maria Grazia OrtoreDepartment of Life and Environmental Sciences, Marche Polytechnic University, Ancona, Italy.
Stefano CasciardiDepartment of Occupational & Environmental Medicine, Epidemiology and Hygiene, National Institute for Insurance Against Accidents at Work (INAIL), Monte Porzio Catone, Italy.
Federica Del ChiericoUnit of Human Microbiome, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Paolo PostorinoCNR-ISC UOS Sapienza and Department of Physics, Sapienza University of Rome, Rome, Italy.
Federico BordiCNR-ISC UOS Sapienza and Department of Physics, Sapienza University of Rome, Rome, Italy.
Andrea MasottiResearch Laboratories, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Istituti di Ricovero e Cura a Carattere Scientifico · ITSapienza University of Rome · ITMarche Polytechnic University · ITBambino Gesù Children's Hospital · ITIstituto Nazionale per l'Assicurazione Contro gli Infortuni sul Lavoro · ITRoma Tre University · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dynamic Light Scattering (DLS), Small Angle X-ray Scattering (SAXS) and Transmission Electron Microscopy (TEM) are physical techniques widely employed to characterize the morphology and the structure of vesicles such as liposomes or human extracellular vesicles (exosomes). Bacterial extracellular vesicles are similar in size to human exosomes, although their function and membrane properties have not been elucidated in such detail as in the case of exosomes. Here, we applied the above cited techniques, in synergy with the thermotropic characterization of the vesicles lipid membrane using a turbidimetric technique to the study of vesicles produced by Gram-negative bacteria (Outer Membrane Vesicles, OMVs) grown at different temperatures. This study demonstrated that our combined approach is useful to discriminate vesicles of different origin or coming from bacteria cultured under different experimental conditions. We envisage that in a near future the techniques employed in our work will be further implemented to discriminate complex mixtures of bacterial vesicles, thus showing great promises for biomedical or diagnostic applications.

Indexed as

dynamic light scatteringgram negative bacteriaouter membrane vesiclesmall-angle X-ray scatteringtransmission electron microscopy

Identifiers

PMID32174900
PMCPMC7056839
OpenAlexW3008267532

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.