ArticleBiosensors & bioelectronics2020
Dielectrophoresis assisted rapid, selective and single cell detection of antibiotic resistant bacteria with G-FETs.
Article in Biosensors & bioelectronics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 86 citations in OpenAlex.
- Discriminating Between Living and Dead Bacteria With a Field-Effect Transistor Based on Nano-Channels.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Point-of-Care Diagnostic Technologies for Antimicrobial Resistance: Principles, Platforms, Clinical Impact, and Future Directions.Diagnostics (Basel, Switzerland) · 2026Review
- Evaluation of single-cell heterogeneity and invasive potential in cancer cells via secreted protease activity assay.Analytica chimica acta · 2025Article
- Electrochemical Biosensing for Antibiotic-Resistant Bacteria: Advances, Challenges, and Future Directions.Micromachines · 2025Review
- Dielectrophoresis-Enhanced Graphene Field-Effect Transistors for Nano-Analyte Sensing.ACS applied materials & interfaces · 2025Article
- Specific gFET-Based Aptasensors for Monitoring of Microbiome Quality: Quantification of the Enteric Health-Relevant Bacterium Roseburia Intestinalis.Advanced healthcare materials · 2025Article
- 2D Materials in Advanced Electronic Biosensors for Point-of-Care Devices.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Transistor-based immunosensor using AuNPs-Ab2-HRP enzyme nanoprobe for the detection of antigen biomarker in human blood.Analytical and bioanalytical chemistry · 2024Article
- Metabolic and Oxidative Stress Effects on the Spectroelectrochemical Behavior of SingleChemical & biomedical imaging · 2023Article
- Current and Future Technologies for the Detection of Antibiotic-Resistant Bacteria.Diagnostics (Basel, Switzerland) · 2023Review
- Rapid Isolation of Low-Level Carbapenem-ResistantBiosensors · 2023Article
- A Review of Carbapenem Resistance inMicroorganisms · 2023Review
- The Integration of Field Effect Transistors to Microfluidic Devices.Micromachines · 2023Review
- New Generation Dielectrophoretic-Based Microfluidic Device for Multi-Type Cell Separation.Biosensors · 2023Article
- Engineered Biosensors for Diagnosing Multidrug Resistance in Microbial and Malignant Cells.Biosensors · 2023Review
- Tutorial on Lateral Dielectrophoretic Manipulations in Microfluidic Systems.IEEE transactions on biomedical circuits and systems · 2023Article
- Two-Dimensional Transition Metal Dichalcogenide Based Biosensors: From Fundamentals to Healthcare Applications.Biosensors · 2023Review
- Micro- and nano-devices for electrochemical sensing.Mikrochimica acta · 2022Review
- Review of Label-Free Monitoring of Bacteria: From Challenging Practical Applications to Basic Research Perspectives.Biosensors · 2022Review
- Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
The rapid increase in antibiotic resistant pathogenic bacteria has become a global threat, which besides the development of new drugs, requires rapid, cheap, scalable, and accurate diagnostics. Label free biosensors relying on electrochemical, mechanical, and mass based detection of whole bacterial cells have attempted to meet these requirements. However, the trade-off between selectivity and sensitivity of such sensors remains a key challenge. In particular, point-of-care diagnostics that are able to reduce and/or prevent unneeded antibiotic prescriptions require highly specific probes with sensitive and accurate transducers that can be miniaturized and multiplexed, and that are easy to operate and cheap. Towards achieving this goal, we present a number of advances in the use of graphene field effect transistors (G-FET) including the first use of peptide probes to electrically detect antibiotic resistant bacteria in a highly specific manner. In addition, we dramatically reduce the needed concentration for detection by employing dielectrophoresis for the first time in a G-FET, allowing us to monitor changes in the Dirac point due to individual bacterial cells. Specifically, we realized rapid binding of bacterial cells to a G-FET by electrical field guiding to the device to realize an overall 3 orders of magnitude decrease in cell-concentration enabling a single-cell detection limit, and 9-fold reduction in needed time to 5 min. Utilizing our new biosensor and procedures, we demonstrate the first selective, electrical detection of the pathogenic bacterial species Staphylococcus aureus and antibiotic resistant Acinetobacter baumannii on a single platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.