ArticleEmerging microbes & infections2020
Identification of chicken CAR homology as a cellular receptor for the emerging highly pathogenic fowl adenovirus 4 via unique binding mechanism.
Article in Emerging microbes & infections, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 42 citations in OpenAlex.
- Outbreak of FAdV-4 infection in blue-breasted quail (Excalfactoria chinensis) reveals novel cross-species transmission of virus in domestic poultry and ornamental pet birds.Poultry science · 2026Article
- Endosomal trafficking factor RAB5c acts as a host restriction factor against FAdV-4 propagation through interaction with ORF1B.Poultry science · 2026Article
- Development of a new TaqMan real-time PCR for detecting Psittacine adenovirus 1 (PsAdV-1).BMC veterinary research · 2026Article
- An optimized sandwich ELISA for quantitative detection of fowl adenovirus serotype 4 (FAdV-4) in chickens.Poultry science · 2026Article
- Aviadenovirus structure: A highly thermostable capsid in the absence of stabilizing proteins.PLoS pathogens · 2025Article
- Overview of the Trending Enteric Viruses and Their Pathogenesis in Intestinal Epithelial Cell Infection.Biomedicines · 2024Review
- The Knob Domain of the Fiber-1 Protein Affects the Replication of Fowl Adenovirus Serotype 4.Microorganisms · 2024Article
- Variation in the affinity of three representative avian adenoviruses for the cellular coxsackievirus and adenovirus receptor.Veterinary research · 2024Article
- Precise location of three novel linear epitopes using the generated monoclonal antibodies against the Knob domain of FAdV-4 surface structural protein, fiber1.Frontiers in cellular and infection microbiology · 2024Article
- Article
- Fowl adenovirus serotype 4 enters leghorn male hepatocellular cells via the clathrin-mediated endocytosis pathway.Veterinary research · 2023Article
- Pathogenicity and epidemiological survey of fowl adenovirus in Shandong Province from 2021 to 2022.Frontiers in microbiology · 2023Article
- An inactivated novel chimeric FAdV-4 containing fiber of FAdV-8b provides full protection against hepatitis-hydropericardium syndrome and inclusion body hepatitis.Veterinary research · 2022Article
- Article
- Hsp70 Inhibits the Replication of Fowl Adenovirus Serotype 4 by Suppressing Viral Hexon with the Assistance of DnaJC7.Journal of virology · 2022Article
- Cellular protein HSC70 promotes fowl adenovirus serotype 4 replication in LMH cells via interacting with viral 100K protein.Poultry science · 2022Article
- The Role of Hexon Amino Acid 188 Varies in Fowl Adenovirus Serotype 4 Strains with Different Virulence.Microbiology spectrum · 2022Article
- FAdV-4 withoutMicrobiology spectrum · 2022Article
- Epidemiology, pathology, prevention, and control strategies of inclusion body hepatitis and hepatitis-hydropericardium syndrome in poultry: A comprehensive review.Frontiers in veterinary science · 2022Review
- Novel monoclonal antibodies against Fiber-1 of duck adenovirus 3 and their B cell epitopes.Frontiers in veterinary science · 2022Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since 2015, the prevalence of severe hepatitis-hydropericardium syndrome, which is caused by the novel genotype fowl adenovirus serotype 4 (FAdV-4), has increased in China and led to considerable economic losses. The replication cycle of FAdV-4, especially the emerging highly pathogenic novel genotype FAdV-4, remains largely unknown. The adenovirus fibre interacts with the cellular receptor as the initial step in adenovirus (AdV) infection. In our previous studies, the complete genome sequence showed that the fibre patterns of FAdV-4 were distinct from all other AdVs. Here, protein-blockage and antibody-neutralization assays were performed to confirm that the novel FAdV-4 short fibre was critical for binding to susceptible leghorn male hepatocellular (LMH) cells. Subsequently, fibre 1 was used as bait to investigate the receptor on LMH cells via mass spectrometry. The chicken coxsackie and adenovirus receptor (CAR) protein was confirmed as the novel FAdV-4 receptor in competition assays. We further identified the D2 domain of CAR (D2-CAR) as the active domain responsible for binding to the short fibre of the novel FAdV-4. Taken together, these findings demonstrate for the first time that the chicken CAR homolog is a cellular receptor for the novel FAdV-4, which facilitates viral entry by interacting with the viral short fibre through the D2 domain. Collectively, these findings provide an in-depth understanding of the mechanisms of the emerging novel genotype FAdV-4 invasion and pathogenesis.
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