Evidence map›Paper›PMID 32173554›Full record

ArticleThe ocular surface2020

Aqueous humor induces lymphatic regression on the ocular surface.

Meng Shi, Lingling Zhang, Eun-Ah Ye, Alice Wang, Guangyu Li, Lu Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The ocular surface, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Acute kidney injury results in long-term alterations of kidney lymphatics in mice.American journal of physiology. Renal physiology · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Meng ShiVision Science Graduate Program, Center for Eye Disease and Development, and School of Optometry, University of California, Berkeley, CA, 94720, USA; Department of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Lingling ZhangVision Science Graduate Program, Center for Eye Disease and Development, and School of Optometry, University of California, Berkeley, CA, 94720, USA.
Eun-Ah YeVision Science Graduate Program, Center for Eye Disease and Development, and School of Optometry, University of California, Berkeley, CA, 94720, USA.
Alice WangVision Science Graduate Program, Center for Eye Disease and Development, and School of Optometry, University of California, Berkeley, CA, 94720, USA.
Guangyu LiVision Science Graduate Program, Center for Eye Disease and Development, and School of Optometry, University of California, Berkeley, CA, 94720, USA.
Lu ChenVision Science Graduate Program, Center for Eye Disease and Development, and School of Optometry, University of California, Berkeley, CA, 94720, USA; The Proctor Foundation for Research in Ophthalmology, University of California, San Francisco, CA, 94143, USA. Electronic address: chenlu@berkeley.edu.
Berkeley Eye Center · USCentral South University · CNUniversity of California, San Francisco · US

Funding

Molecular Regulation of Lymphangiogenesis in Corneal AlloimmunityR01EY017392 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHEN, LU · 2007 to 2020
$5.7M
Novel characterization and therapeutic intervention of Schlemm's canal for glaucoma treatmentR01EY028995 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHEN, LU · 2018 to 2021
$1.6M
NEI NIH HHS R01 EY017392NEI NIH HHS R01 EY028995
6 · The paper itself

Abstract

purposeThis study is to investigate the potential effect of aqueous humor on already formed lymphatic vessels of the ocular surface including the conjunctiva and the cornea.

methodsAqueous humor harvested from fresh bovine or murine eyeballs were used in the study. It was injected into the subconjunctival space of Prox-1-GFP (green fluorescent protein) transgenic mice. Pre-existing conjunctival lymphatics were observed in vivo using our advanced live imaging system. Additionally, ex vivo tissue cultures were performed in aqueous humor with normal conjunctival tissues or inflamed corneas with newly formed lymphatic vessels. Time lapse images were taken by an advanced live cell imaging system with an incubator. Moreover, human primary microdermal lymphatic endothelial cell culture system was employed to evaluate the effect of aqueous humor on lymphatic tube regression in vitro.

resultsAqueous humor induced lymphatic regression in both normal conjunctiva and inflamed corneas. It also led to the regression of formed lymphatic tubes by the lymphatic endothelial cells in vitro.

conclusionsThis study provides the first direct and real time live imaging evidence showing that aqueous humor induces lymphatic regression. Further investigation promises for divulging new mechanisms and therapeutic strategies to treat lymphatic diseases that occur both inside and outside the eye.

Indexed as

Aqueous HumorLymphatic VesselsAnimalsCattleCorneaEndothelial CellsMiceMice, Inbred C57BLAqueous humorConjunctivaCorneaEndothelial cell functionLive imagingLymphatic vesselOcular surface

Identifiers

PMID32173554
PMCPMC8099855
OpenAlexW3012020151

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.