ArticleBMC genomics2020
Systematic analysis of the basic/helix-loop-helix (bHLH) transcription factor family in pummelo (Citrus grandis) and identification of the key members involved in the response to iron deficiency.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 54 citations in OpenAlex.
- Genome-wide identification and expression profiling of the bHLH transcription factor family under salt stress and hormonal treatments in cowpea (Vigna unguiculata).Scientific reports · 2026Article
- Genome-Wide Identification and Evolutionary Analysis of the bHLH Transcription Factor Family inInternational journal of molecular sciences · 2026Article
- Genome-wide identification and expression analysis of salt-responsive bHLH transcription factors in the wheat (Frontiers in plant science · 2026Article
- Molecular Mechanism by Which the BrFIT2-BrbHLH-BrIRT1 Module Synergistically Regulates Iron Absorption in Brassica rapa ssp. Pekinensis.Plant biotechnology journal · 2025Article
- Whole-genome identification and functional analysis of grape bHLH transcription factors: new insights into the regulation of monoterpene biosynthesis.Frontiers in plant science · 2025Article
- A bHLH Transcription Factor Confers Salinity Stress Tolerance inPlant direct · 2024Article
- Functions and Regulatory Mechanisms of bHLH Transcription Factors during the Responses to Biotic and Abiotic Stresses in Woody Plants.Plants (Basel, Switzerland) · 2024Review
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- Automatic annotation of the bHLH gene family in plants.BMC genomics · 2023Article
- Genome-Wide Identification and Characterization of thePlants (Basel, Switzerland) · 2023Article
- Comprehensive Analysis of bHLH Transcription Factors inInternational journal of molecular sciences · 2023Article
- Comprehensive Identification and Analyses of the GRF Gene Family in the Whole-Genome of Four Juglandaceae Species.International journal of molecular sciences · 2022Article
- Iron redistribution induces oxidative burst and resistance in maize against Curvularia lunata.Planta · 2022Article
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- Genome-Wide Characterization and Analysis of the bHLH Transcription Factor Family inFrontiers in genetics · 2022Article
- MYB308-mediated transcriptional activation of plasma membrane HHorticulture research · 2022Article
- Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance.International journal of molecular sciences · 2021Review
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- Automatic Identification of Players in the Flavonoid Biosynthesis with Application on the Biomedicinal PlantPlants (Basel, Switzerland) · 2020Article
- Phytochemical and Therapeutic Potential ofJournal of evidence-based integrative medicineReview
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIron (Fe) deficiency is a common problem in citrus production. As the second largest superfamily of transcription factors (TFs), the basic/helix-loop-helix (bHLH) proteins have been shown to participate in the regulation of Fe homeostasis and a series of other biological and developmental processes in plants. However, this family of members in citrus and their functions in citrus Fe deficiency are still largely unknown.
resultsIn this study, we identified a total of 128 CgbHLHs from pummelo (Citrus grandis) genome that were classified into 18 subfamilies by phylogenetic comparison with Arabidopsis thaliana bHLH proteins. All of these CgbHLHs were randomly distributed on nine known (125 genes) and one unknown (3 genes) chromosomes, and 12 and 47 of them were identified to be tandem and segmental duplicated genes, respectively. Sequence analysis showed detailed characteristics of their intron-exon structures, bHLH domain and conserved motifs. Gene ontology (GO) analysis suggested that most of CgbHLHs were annotated to the nucleus, DNA-binding transcription factor activity, response to abiotic stimulus, reproduction, post-embryonic development, flower development and photosynthesis. In addition, 27 CgbHLH proteins were predicted to have direct or indirect protein-protein interactions. Based on GO annotation, RNA sequencing data in public database and qRT-PCR results, several of CgbHLHs were identified as the key candidates that respond to iron deficiency.
conclusionsIn total, 128 CgbHLH proteins were identified from pummelo, and their detailed sequence and structure characteristics and putative functions were analyzed. This study provides comprehensive information for further functional elucidation of CgbHLH genes in citrus.
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