ArticlePlant biotechnology journal2020
Manipulation of ZDS in tomato exposes carotenoid- and ABA-specific effects on fruit development and ripening.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 68 citations in OpenAlex.
- Bioconversion of carotenoids into high-value crocins using a marine sponge carotenoid cleavage dioxygenase.The New phytologist · 2026Article
- Decoding Isoprenoid Transcript-Metabolite Interactions in Carotenoid Tomato Fruit Mutants Uncovers Novel Metabolic Cross-Links.International journal of molecular sciences · 2026Article
- The carotenoids metabolism during tomato fruit ripening: insights into multi-level regulatory network.BMC plant biology · 2026Review
- What metabolomics has taught us about tomato fruit ripening and quality.Journal of experimental botany · 2025Review
- Genome-wide association study of carotenoids in maize kernel.The plant genome · 2024Article
- Overlapping and specialized roles of tomato phytoene synthases in carotenoid and abscisic acid production.Plant physiology · 2023Article
- Effects of sgRNAs, Promoters, and Explants on the Gene Editing Efficiency of the CRISPR/Cas9 System in Chinese Kale.International journal of molecular sciences · 2023Article
- Genome-wide identification of the geranylgeranyl pyrophosphate synthase (GGPS) gene family involved in chlorophyll synthesis in cotton.BMC genomics · 2023Article
- The transcription factor IbNAC29 positively regulates the carotenoid accumulation in sweet potato.Horticulture research · 2023Article
- Abscisic acid biosynthesis, metabolism and signaling in ripening fruit.Frontiers in plant science · 2023Review
- Spatiotemporal dynamics of the tomato fruit transcriptome under prolonged water stress.Plant physiology · 2022Article
- SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato.Plant biotechnology journal · 2022Article
- Comparative transcriptomics of wild and commercial Citrus during early ripening reveals how domestication shaped fruit gene expression.BMC plant biology · 2022Article
- The Fruit Proteome Response to the Ripening Stages in Three Tomato Genotypes.Plants (Basel, Switzerland) · 2022Article
- The metabolic changes that effect fruit quality during tomato fruit ripening.Molecular horticulture · 2022Review
- Down-regulation ofFrontiers in plant science · 2022Article
- Quantitative Transcriptomic and Proteomic Analysis of Fruit Development and Ripening in Watermelon (Frontiers in plant science · 2022Article
- "Omics" insights into plastid behavior toward improved carotenoid accumulation.Frontiers in plant science · 2022Review
- Carotenoid Biosynthetic Genes in Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis.Genes · 2021Article
- Carotenoids and Apocarotenoids in Planta: Their Role in Plant Development, Contribution to the Flavour and Aroma of Fruits and Flowers, and Their Nutraceutical Benefits.Plants (Basel, Switzerland) · 2021Review
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spontaneous mutations in fruit-specific carotenoid biosynthetic genes of tomato (Solanum lycopersicum) have led to improved understanding of ripening-associated carotenogenesis. Here, we confirm that ZDS is encoded by a single gene in tomato transcriptionally regulated by ripening transcription factors RIN, NOR and ethylene. Manipulation of ZDS was achieved through transgenic repression and heterologous over-expression in tomato. CaMV 35S-driven RNAi repression inhibited carotenoid biosynthesis in all aerial tissues examined resulting in elevated levels of ζ-carotene isomers and upstream carotenoids, while downstream all trans-lycopene and subsequent photoprotective carotenes and xanthophylls were diminished. Consequently, immature fruit displayed photo-bleaching consistent with reduced levels of the photoprotective carotenes and developmental phenotypes related to a reduction in the carotenoid-derived phytohormone abscisic acid (ABA). ZDS-repressed ripe fruit was devoid of the characteristic red carotenoid, all trans-lycopene and displayed brilliant yellow pigmentation due to elevated 9,9' di-cis-ζ-carotene. Over-expression of the Arabidopsis thaliana ZDS (AtZDS) gene bypassed endogenous co-suppression and revealed ZDS as an additional bottleneck in ripening-associated carotenogenesis of tomato. Quantitation of carotenoids in addition to multiple ripening parameters in ZDS-altered lines and ABA-deficient fruit-specific carotenoid mutants was used to separate phenotypic consequences of ABA from other effects of ZDS manipulation and reveal a unique and dynamic ζ-carotene isomer profile in ripe fruit.
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