Evidence map›Paper›PMID 32170988›Full record

ArticleTraffic (Copenhagen, Denmark)2020

Super-beacons: Open-source probes with spontaneous tuneable blinking compatible with live-cell super-resolution microscopy.

Pedro M Pereira, Nils Gustafsson, Mark Marsh, Musa M Mhlanga, Ricardo Henriques

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Traffic (Copenhagen, Denmark), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Pedro M PereiraMRC-Laboratory for Molecular Cell Biology, University College London, London, UK.
Nils GustafssonMRC-Laboratory for Molecular Cell Biology, University College London, London, UK.
Mark MarshMRC-Laboratory for Molecular Cell Biology, University College London, London, UK.
Musa M MhlangaInstitute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.
Ricardo HenriquesMRC-Laboratory for Molecular Cell Biology, University College London, London, UK.

Funding

Biotechnology and Biological Sciences Research Council BB/M022374/1Biotechnology and Biological Sciences Research Council BB/P027431/1Biotechnology and Biological Sciences Research Council BB/R000697/1Engineering and Physical Sciences Research Council EP/L504889/1Medical Research Council MR/K015826/1Wellcome TrustWellcome Trust 203276/Z/16/Z
6 · The paper itself

Abstract

Localization-based super-resolution microscopy relies on the detection of individual molecules cycling between fluorescent and non-fluorescent states. These transitions are commonly regulated by high-intensity illumination, imposing constrains to imaging hardware and producing sample photodamage. Here, we propose single-molecule self-quenching as a mechanism to generate spontaneous photoswitching. To demonstrate this principle, we developed a new class of DNA-based open-source super-resolution probes named super-beacons, with photoswitching kinetics that can be tuned structurally, thermally and chemically. The potential of these probes for live-cell compatible super-resolution microscopy without high-illumination or toxic imaging buffers is revealed by imaging interferon inducible transmembrane proteins (IFITMs) at sub-100 nm resolutions.

Indexed as

BlinkingDNAMicroscopy, FluorescenceFluorescent DyesDNAFluorescent DyesDNAfluorescence microscopyfluorescence quenchinglive-cellmolecular-beaconssuper-resolution

Identifiers

PMID32170988
PMCPMC7643006

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.