ArticleInsect biochemistry and molecular biology2020
Characterization of an adulticidal and larvicidal interfering RNA pesticide that targets a conserved sequence in mosquito G protein-coupled dopamine 1 receptor genes.
Article in Insect biochemistry and molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Soda-baited RNAi yeast insecticides as effective Attractive Targeted Sugar Baits (ATSBs) for mosquito control.PLoS neglected tropical diseases · 2026Article
- Targeting the Highly Invasive Malaria Vector Anopheles stephensi using Yeast RNAi Pesticides.Research square · 2026Article
- Bibliometric trends and emerging frontiers in RNA interference research for mosquito control (2010-2025).Frontiers in insect science · 2026Review
- Control of Aedes albopictus populations by silencing of the vesicular GABA transporter (vgat) and the vesicular monoamine transporter (vmat) genes using recombinant Chlorella shRNA.Parasites & vectors · 2025Article
- Challenges of Robust RNAi-Mediated Gene Silencing inInternational journal of molecular sciences · 2024Article
- Combination of computational techniques and RNAi reveal targets in Anopheles gambiae for malaria vector control.PloS one · 2024Article
- Characterization of a novel RNAi yeast insecticide that silences mosquito 5-HT1 receptor genes.Scientific reports · 2023Article
- Targeting Mosquitoes through Generation of an Insecticidal RNAi Yeast Strain Using Cas-CLOVER and Super PiggyBac Engineering inJournal of fungi (Basel, Switzerland) · 2023Article
- Maximizing the Potential of Attractive Targeted Sugar Baits (ATSBs) for Integrated Vector Management.Insects · 2023Review
- Mosquito gene targeted RNAi studies for vector control.Functional & integrative genomics · 2023Review
- Oral RNAi for Gene Silencing in Mosquitoes: From the Bench to the Field.Cold Spring Harbor protocols · 2022Article
- A Conserved Female-Specific Requirement for thePathogens (Basel, Switzerland) · 2022Article
- RNAi-based pest control: Production, application and the fate of dsRNA.Frontiers in bioengineering and biotechnology · 2022Review
- A Yeast RNA-Interference Pesticide Targeting theInsects · 2021Article
- A Broad-Based Mosquito Yeast Interfering RNA Pesticide TargetingPathogens (Basel, Switzerland) · 2021Article
- G-Protein Coupled Receptors (GPCRs) in Insects-A Potential Target for New Insecticide Development.Molecules (Basel, Switzerland) · 2021Review
- G-Protein Coupled Receptors (GPCRs): Signaling Pathways, Characterization, and Functions in Insect Physiology and Toxicology.International journal of molecular sciences · 2021Review
- Article
- Delivery Methods for RNAi in Mosquito Larvae.Journal of insect science (Online) · 2020Review
- Community acceptance of yeast interfering RNA larvicide technology for control of Aedes mosquitoes in Trinidad.PloS one · 2020Article
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Authors and funding
12 authors at 1 institution in 2 countries.
Funding
Abstract
G protein-coupled receptors (GPCRs), key regulators of a variety of critical biological processes, are attractive targets for insecticide development. Given the importance of these receptors in many organisms, including humans, it is critical that novel pesticides directed against GPCRs are designed to be species-specific. Here, we present characterization of an interfering RNA pesticide (IRP) targeting the mosquito GPCR-encoding dopamine 1 receptor (dop1) genes. A small interfering RNA corresponding to dop1 was identified in a screen for IRPs that kill Aedes aegypti during both the adult and larval stages. The 25 bp sequence targeted by this IRP is conserved in the dop1 genes of multiple mosquito species, but not in non-target organisms, indicating that it could function as a biorational mosquito insecticide. Aedes aegypti adults treated through microinjection or attractive toxic sugar bait delivery of small interfering RNA corresponding to the target site exhibited severe neural and behavioral defects and high levels of adult mortality. Likewise, A. aegypti larval consumption of dried inactivated yeast tablets prepared from a Saccharomyces cerevisiae strain engineered to express short hairpin RNA corresponding to the dop1 target site resulted in severe neural defects and larval mortality. Aedes albopictus and Anopheles gambiae adult and larval mortality was also observed following treatment with dop1 IRPs, which were not toxic to non-target arthropods. The results of this investigation indicate that dop1 IRPs can be used for species-specific targeting of dop1 GPCRs and may represent a new biorational strategy for control of both adult and larval mosquitoes.
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