Evidence map›Paper›PMID 32169215›Full record

ArticleCell2020

Initiation of Parental Genome Reprogramming in Fertilized Oocyte by Splicing Kinase SRPK1-Catalyzed Protamine Phosphorylation.

Lan-Tao Gou, Do-Hwan Lim, Wubin Ma, Brandon E Aubol, Yajing Hao, Xin Wang, Jun Zhao, Zhengyu Liang, Changwei Shao, Xuan Zhang and 10 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
6.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 102 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
  10. Protamine lacunae preserve the paternal chromatin landscape in sperm.bioRxiv : the preprint server for biology · 2025
    Article
  11. Article
  12. Review
  13. Article
  14. Adverse Effects of Nicotine on Human Sperm Nuclear Proteins.The world journal of men's health · 2025
    Review
  15. Review
  16. The Mammalian Oocyte: A Central Hub for Cellular Reprogramming and Stemness.Stem cells and cloning : advances and applications · 2025
    Review
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 5 institutions in 2 countries.

Lan-Tao GouDepartment of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Do-Hwan LimDepartment of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Wubin MaHoward Hughes Medical Institute, Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Brandon E AubolDepartment of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA.
Yajing HaoDepartment of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Xin WangState Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences-University of Chinese Academy of Sciences, Shanghai 200031, China.
Jun ZhaoTransgenic and Knockout Mouse Core, University of California, San Diego, La Jolla, CA 92093, USA.
Zhengyu LiangDepartment of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Changwei ShaoDepartment of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Xuan ZhangDepartment of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Fan MengDepartment of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Hairi LiDepartment of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Xiaorong ZhangInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Ruiming XuInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Dangsheng LiShanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
Michael G RosenfeldHoward Hughes Medical Institute, Department of Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Pamela L MellonTransgenic and Knockout Mouse Core, University of California, San Diego, La Jolla, CA 92093, USA; Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
Joseph A AdamsDepartment of Pharmacology, University of California, San Diego, La Jolla, CA 92093, USA.
Mo-Fang LiuState Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences-University of Chinese Academy of Sciences, Shanghai 200031, China.
Xiang-Dong FuDepartment of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: xdfu@ucsd.edu.
University of California, San Diego · USChinese Academy of Sciences · CNHoward Hughes Medical Institute · USCenter for Excellence in Molecular Cell Science · CNUniversity of Chinese Academy of Sciences · CN

Funding

VIRAL MALIGNANCYP30CA023100 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jesse Nodora · 1985 to 2026
$124.9M
Yeast Genetics and Stress Response Genes-BiomedicalP42ES010337 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TUKEY, ROBERT H · 2000 to 2022
$69.8M
Transgenic & Knock-out MouseP30DK063491 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Andrea L Hevener · 2003 to 2026
$40.4M
Functional RNA elements in the human genomeR01HG004659 · NHGRI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Kevin Daniel Corbett, Eugene Wei-Ming Yeo · 2008 to 2026
$12.4M
FUNCTION &REGULATION OF THE HUMAN SPLICING FACTOR SC35R01GM049369 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FU, XIANG-DONG · 1993 to 2018
$7.2M
Role of protein phosphorylation in RNA splicingR01GM067969 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ADAMS, JOSEPH · 2004 to 2024
$6.4M
Regulation of RNA metabolism and cell growth control by SR protein kinasesR01GM052872 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DOWDY, STEVEN F · 1996 to 2019
$5.4M
SRPK1 regulation of the protamine-to-histone exchange in early embryonic developmentK99HD094901 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GOU, LANTAO · 2018 to 2019
$264k
NCI NIH HHS P30 CA023100NHGRI NIH HHS R01 HG004659NICHD NIH HHS K99 HD094901NIDDK NIH HHS P30 DK063491NIEHS NIH HHS P42 ES010337NIGMS NIH HHS R01 GM049369NIGMS NIH HHS R01 GM052872NIGMS NIH HHS R01 GM067969
6 · The paper itself

Abstract

The paternal genome undergoes a massive exchange of histone with protamine for compaction into sperm during spermiogenesis. Upon fertilization, this process is potently reversed, which is essential for parental genome reprogramming and subsequent activation; however, it remains poorly understood how this fundamental process is initiated and regulated. Here, we report that the previously characterized splicing kinase SRPK1 initiates this life-beginning event by catalyzing site-specific phosphorylation of protamine, thereby triggering protamine-to-histone exchange in the fertilized oocyte. Interestingly, protamine undergoes a DNA-dependent phase transition to gel-like condensates and SRPK1-mediated phosphorylation likely helps open up such structures to enhance protamine dismissal by nucleoplasmin (NPM2) and enable the recruitment of HIRA for H3.3 deposition. Remarkably, genome-wide assay for transposase-accessible chromatin sequencing (ATAC-seq) analysis reveals that selective chromatin accessibility in both sperm and MII oocytes is largely erased in early pronuclei in a protamine phosphorylation-dependent manner, suggesting that SRPK1-catalyzed phosphorylation initiates a highly synchronized reorganization program in both parental genomes.

Indexed as

AnimalsCell Cycle ProteinsCell NucleusChromatinChromatin Assembly and DisassemblyFertilizationHistonesMaleMiceMice, Inbred BALB CMice, Inbred C57BLMice, KnockoutOocytesPhosphorylationProtamine KinaseProtaminesCell Cycle ProteinsChromatinHistonesPRM1 protein, humanProtamine KinaseProtaminesProtein Serine-Threonine KinasesSRPK1 protein, humanTranscription Factorsfertilizationgenome reprogramminghistone chaperonesphosphorylationprotamineprotamine-to-histone exchangeSR protein-specific kinasezygotic development

Identifiers

PMID32169215
PMCPMC7190278
OpenAlexW3012283905

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.