Evidence map›Paper›PMID 32169079›Full record

ArticleMicrobial cell factories2020

Resolution of alkaloid racemate: a novel microbial approach for the production of enantiopure lupanine via industrial wastewater valorization.

Stella Parmaki, Argyro Tsipa, Marlen I Vasquez, João M J Gonçalves, Ioanna Hadjiadamou, Frederico C Ferreira, Carlos A M Afonso, Chrysoulla Drouza, Michalis Koutinas

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In one paragraph

Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Stella ParmakiDepartment of Chemical Engineering, Cyprus University of Technology, 30 Archbishop Kyprianou Str., 3036, Limassol, Cyprus.
Argyro TsipaCivil and Environmental Engineering, University of Cyprus, 75 Kallipoleos Str., 1678, Nicosia, Cyprus.
Marlen I VasquezDepartment of Chemical Engineering, Cyprus University of Technology, 30 Archbishop Kyprianou Str., 3036, Limassol, Cyprus.
João M J GonçalvesResearch Institute for Medicines (iMed. ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
Ioanna HadjiadamouDepartment of Chemistry, University of Cyprus, P.O. Box 20537, 1678, Nicosia, Cyprus.
Frederico C FerreiraInstitute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal.
Carlos A M AfonsoResearch Institute for Medicines (iMed. ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisbon, Portugal.
Chrysoulla DrouzaDepartment of Agricultural Sciences, Biotechnology and Food Science, Cyprus University of Technology, 30 Archbishop Kyprianou Str., 3036, Limassol, Cyprus.
Michalis KoutinasDepartment of Chemical Engineering, Cyprus University of Technology, 30 Archbishop Kyprianou Str., 3036, Limassol, Cyprus. michail.koutinas@cut.ac.cy.ORCID http://orcid.org/0000-0002-5371-4280

Funding

Cyprus Research and Innovation Foundation ΚΟΙΝΑ/ΠΚΠ-WATER/0315/14Fundação para a Ciência e a Tecnologia WaterJPI/0001/2014Fundação para a Ciência e a Tecnologia WaterJPI/0002/2014Fundação para a Ciência e a Tecnologia WaterJPI/0003/2014
6 · The paper itself

Abstract

backgroundLupanine is a plant toxin contained in the wastewater of lupine bean processing industries, which could be used for semi-synthesis of various novel high added-value compounds. This paper introduces an environmental friendly process for microbial production of enantiopure lupanine.

resultsPreviously isolated P. putida LPK411, R. rhodochrous LPK211 and Rhodococcus sp. LPK311, holding the capacity to utilize lupanine as single carbon source, were employed as biocatalysts for resolution of racemic lupanine. All strains achieved high enantiomeric excess (ee) of L-(-)-lupanine (> 95%), while with the use of LPK411 53% of the initial racemate content was not removed. LPK411 fed with lupanine enantiomers as single substrates achieved 92% of D-(+)-lupanine biodegradation, whereas L-(-)-lupanine was not metabolized. Monitoring the transcriptional kinetics of the luh gene in cultures supplemented with the racemate as well as each of the enantiomers supported the enantioselectivity of LPK411 for D-(+)-lupanine biotransformation, while (trans)-6-oxooctahydro-1H-quinolizine-3-carboxylic acid was detected as final biodegradation product from D-(+)-lupanine use. Ecotoxicological assessment demonstrated that lupanine enantiomers were less toxic to A. fischeri compared to the racemate exhibiting synergistic interaction.

conclusionsThe biological chiral separation process of lupanine presented here constitutes an eco-friendly and low-cost alternative to widely used chemical methods for chiral separation.

Indexed as

BiotransformationFood IndustryLupinusPseudomonas putidaRhodococcusSparteineStereoisomerismWastewaterlupanineSparteineWastewaterEcotoxicological assessmentEnantiomeric excessEnantiomersGene expressionLupaninePseudomonas putida LPK411Quantitative real-time PCR

Identifiers

PMID32169079
PMCPMC7071741

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LicenceCC BY
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.