ArticleMicrobial cell factories2020
Improving L-serine formation by Escherichia coli by reduced uptake of produced L-serine.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 29 citations in OpenAlex.
- L-serine at the crossroads of microbiota, intestinal health, and disorders.Communications biology · 2026Review
- Harnessing prokaryotic amino acid transporters for metabolic engineering: mechanisms and biotechnological applications.Synthetic and systems biotechnology · 2026Review
- Enhanced l-serine synthesis inSynthetic and systems biotechnology · 2025Article
- Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration.Biotechnology for biofuels and bioproducts · 2024Article
- Nitrogen assimilation bymBio · 2024Article
- Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments.Synthetic and systems biotechnology · 2023Article
- Characterization of thermostable serine hydroxymethyltransferase for β-hydroxy amino acids synthesis.Amino acids · 2023Article
- Bioprocess Engineering, Transcriptome, and Intermediate Metabolite Analysis of L-Serine High-YieldingMicroorganisms · 2022Article
- Transcriptome Analysis Reveals Potential Mechanisms of L-Serine Production byFoods (Basel, Switzerland) · 2022Article
- GeneFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundMicrobial de novo production of L-serine, which is widely used in a range of cosmetic and pharmaceutical products, has attracted increasing attention due to its environmentally friendly characteristics. Previous pioneering work mainly focused on L-serine anabolism; however, in this study, it was found that L-serine could be reimported through the L-serine uptake system, thus hampering L-serine production.
resultTo address this challenge, engineering via deletion of four genes, namely, sdaC, cycA, sstT and tdcC, which have been reported to be involved in L-serine uptake in Escherichia coli, was first carried out in the L-serine producer E. coli ES. Additionally, the effects of these genes on L-serine uptake activity and L-serine production were investigated. The data revealed an abnormal phenomenon regarding serine uptake activity. The serine uptake activity of the ΔsdaC mutant was 0.798 nmol min
conclusionThe results indicated that reuptake of L-serine impairs its production and that an engineered cell with reduced uptake can address this problem and improve the production of L-serine in E. coli.
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Registered trials
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