ArticleBMC microbiology2020
Modified high-throughput Nile red fluorescence assay for the rapid screening of oleaginous yeasts using acetic acid as carbon source.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Sulfo-phospho-vanillin method for screening Aurantiochytrium strains with high docosahexaenoic acid levels.AMB Express · 2025Article
- Enhancing Chitin Production as a Fermentation Byproduct through a Genetic Toolbox That Activates the Cell Wall Integrity Response.ACS synthetic biology · 2025Article
- Oleaginous fungi: a promising source of biofuels and nutraceuticals with enhanced lipid production strategies.Archives of microbiology · 2024Review
- Nile red-based lipid fluorometry protocol and its use for statistical optimization of lipids in oleaginous yeasts.Applied microbiology and biotechnology · 2023Article
- Optimization of a hybrid bacterial/Metabolic engineering communications · 2023Article
- Production of short-chain fatty acids (SCFAs) as chemicals or substrates for microbes to obtain biochemicals.Biotechnology for biofuels and bioproducts · 2023Review
- Yarrowia lipolytica produces lipid-rich biomass in medium mimicking lignocellulosic biomass hydrolysate.Applied microbiology and biotechnology · 2023Article
- Production of Single-Cell Oil from a Local IsolateInternational journal of biomaterials · 2023Article
- Discovery of Oleaginous Yeast from Mountain Forest Soil in Thailand.Journal of fungi (Basel, Switzerland) · 2022Article
- Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains.Biotechnology for biofuels and bioproducts · 2022Article
- Microbes: A Hidden Treasure of Polyunsaturated Fatty Acids.Frontiers in nutrition · 2022Review
- The Oleaginous YeastBiology · 2021Article
- Yeast Cells in Microencapsulation. General Features and Controlling Factors of the Encapsulation Process.Molecules (Basel, Switzerland) · 2021Review
- Nile Red Incubation Time Before Reading Fluorescence Greatly Influences the Yeast Neutral Lipids Quantification.Frontiers in microbiology · 2021Article
- Single Cell Oil Production by Oleaginous Yeasts Grown in Synthetic and Waste-Derived Volatile Fatty Acids.Microorganisms · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundOver the last years oleaginous yeasts have been studied for several energetic, oleochemical, medical and pharmaceutical purposes. However, only a small number of yeasts are known and have been deeply exploited. The search for new isolates with high oleaginous capacity becomes imperative, as well as the use of alternative and ecological carbon sources for yeast growth.
resultsIn the present study a high-throughput screening comprising 366 distinct yeast isolates was performed by applying an optimised protocol based on two approaches: (I) yeast cultivation on solid medium using acetic acid as carbon source, (II) neutral lipid estimation by fluorimetry using the lipophilic dye Nile red.
conclusionsResults showed that, with the proposed methodology, the oleaginous potential of yeasts with broad taxonomic diversity and variety of growth characteristics was discriminated. Furthermore, this work clearly demonstrated the association of the oleaginous yeast character to the strain level, contrarily to the species-level linkage, as usually stated.
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