ReviewGenes2020
Standards for Methods Utilizing Environmental DNA for Detection of Fish Species.
Review in Genes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Review
- Beyond Species Richness: A Statistically Rigorous Comparison of eDNA and Conventional Fish Sampling Methods.Environmental science & technology · 2026Article
- Article
- Article
- Environmental DNA Based Assessment of Fish Diversity in the Yarlung Zangbo River, Tibetan Plateau.Ecology and evolution · 2025Article
- QuickConc: A Rapid, Efficient, and Power-Free eDNA Concentration Method With Cationic-Assisted Capture.Ecology and evolution · 2025Article
- Environmental DNA Reveals the Fish Community Structure Exhibited Instability and Trend of Miniaturization in the Xijiang River Basin of the Guizhou.Ecology and evolution · 2025Article
- Navigating Methodological Trade-Offs in eDNA Metabarcoding Biodiversity Monitoring: Insights From a Mediterranean Watershed.Molecular ecology resources · 2025Article
- The Long and the Short of It: Nanopore-Based eDNA Metabarcoding of Marine Vertebrates Works; Sensitivity and Species-Level Assignment Depend on Amplicon Lengths.Molecular ecology resources · 2025Article
- Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems.Biology · 2025Article
- No pump, no problem: evaluating passive eDNA sampling for marine biomonitoring of a nuisance macroalga.PeerJ · 2025Article
- Insights into the representativeness of biodiversity assessment in large reservoir through eDNA metabarcoding.PloS one · 2025Article
- Enhancing African coelacanth monitoring using environmental DNA.Biology letters · 2024Article
- Multi-method survey rediscovers critically endangered species and strengthens Madagascar's freshwater fish conservation.Scientific reports · 2024Article
- Advances in environmental DNA monitoring: standardization, automation, and emerging technologies in aquatic ecosystems.Science China. Life sciences · 2024Review
- Real-Time Monitoring of a Nucleic Acid Amplification Reaction Using a Mass Sensor Based on a Quartz-Crystal Microbalance.Biosensors · 2024Article
- Meiofauna at a tropical sandy beach in the SW Atlantic: the influence of seasonality on diversity.PeerJ · 2024Article
- Sustainable fisheries management through reliable restocking and stock enhancement evaluation with environmental DNA.Scientific reports · 2023Article
- Detection of vertebrates from natural and artificial inland water bodies in a semi-arid habitat using eDNA from filtered, swept, and sediment samples.Ecology and evolution · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Environmental DNA (eDNA) techniques are gaining attention as cost-effective, non-invasive strategies for acquiring information on fish and other aquatic organisms from water samples. Currently, eDNA approaches are used to detect specific fish species and determine fish community diversity. Various protocols used with eDNA methods for aquatic organism detection have been reported in different eDNA studies, but there are no general recommendations for fish detection. Herein, we reviewed 168 papers to supplement and highlight the key criteria for each step of eDNA technology in fish detection and provide general suggestions for eliminating detection errors. Although there is no unified recommendation for the application of diverse eDNA in detecting fish species, in most cases, 1 or 2 L surface water collection and eDNA capture on 0.7-μm glass fiber filters followed by extraction with a DNeasy Blood and Tissue Kit or PowerWater DNA Isolation Kit are useful for obtaining high-quality eDNA. Subsequently, species-specific quantitative polymerase chain reaction (qPCR) assays based on mitochondrial cytochrome b gene markers or eDNA metabarcoding based on both
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