ArticleNano letters2020
High-Speed Super-Resolution Imaging Using Protein-Assisted DNA-PAINT.
Article in Nano letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed.
- Stoichiometrically Defined Antibody-DNA Conjugates for Quantitative Super-Resolution Imaging.Nano letters · 2026Article
- Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells.bioRxiv : the preprint server for biology · 2026Article
- Advanced Imaging for Live-Cell Spatiotemporal Monitoring: Technologies and Applications.Research (Washington, D.C.) · 2026Review
- Single Molecule Kinetic Fingerprinting of Glycans on IgA1 Antibodies.Analytical chemistry · 2025Article
- High-Throughput Single-Molecule Microscopy with Adaptable Spatial Resolution Using Exchangeable Oligonucleotide Labels.ACS nano · 2025Article
- Decoding aptamer-protein binding kinetics for continuous biosensing using single-molecule techniques.Science advances · 2025Article
- A conditional protein diffusion model generates artificial programmable endonuclease sequences with enhanced activity.Cell discovery · 2024Article
- Visualizing the Heterogeneity in Homogeneous Supramolecular Polymers.Journal of the American Chemical Society · 2024Article
- Improved Imaging Surface for Quantitative Single-Molecule Microscopy.ACS applied materials & interfaces · 2024Article
- Full-length single-molecule protein fingerprinting.Nature nanotechnology · 2024Article
- MnChemical science · 2024Article
- Article
- Multiplexed and Millimeter-Scale Fluorescence Nanoscopy of Cells and Tissue Sections via Prism-Illumination and Microfluidics-Enhanced DNA-PAINT.Chemical & biomedical imaging · 2023Article
- Prokaryotic Argonaute Proteins: A New Frontier in Point-of-Care Viral Diagnostics.International journal of molecular sciences · 2023Review
- Deep learning-enabled fast DNA-PAINT imaging in cells.Biophysics reports · 2023Article
- smFRET Detection of Cis and Trans DNA Interactions by the BfiI Restriction Endonuclease.The journal of physical chemistry. B · 2023Article
- Argonaute protein-based nucleic acid detection technology.Frontiers in microbiology · 2023Review
- Programmable RNA targeting by bacterial Argonaute nucleases with unconventional guide binding and cleavage specificity.Nature communications · 2022Article
- Fluorogenic DNA-PAINT for faster, low-background super-resolution imaging.Nature methods · 2022Article
- Bleaching-Resistant Super-Resolution Fluorescence Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Super-resolution imaging allows for the visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA point accumulation in nanoscale topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of the imager strands. Here we report on a method where imager strands are loaded into a protein, Argonaute (Ago), which allows for faster binding. Ago preorders the DNA imager strand into a helical conformation, allowing for 10 times faster target binding. Using a 2D DNA origami structure, we demonstrate that Ago-assisted DNA-PAINT (Ago-PAINT) can speed up the current DNA-PAINT technique by an order of magnitude, while maintaining the high spatial resolution. We envision this tool to be useful for super-resolution imaging and other techniques that rely on nucleic acid interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.