ArticlePLoS biology2020
Single-cell in vivo imaging of cellular circadian oscillators in zebrafish.
Article in PLoS biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Zebrafish as a translational filter for natural sleep modulators.Frontiers in nutrition · 2026Review
- Substance P and dopamine form a "push-pull" system that diurnally regulates retinal gain.Current biology : CB · 2024Article
- Article
- Repression of latent NF-κB enhancers by PDX1 regulates β cell functional heterogeneity.Cell metabolism · 2024Article
- Her6 and Prox1a are novel regulators of photoreceptor regeneration in the zebrafish retina.PLoS genetics · 2023Article
- Heterogeneity in quiescent Müller glia in the uninjured zebrafish retina drive differential responses following photoreceptor ablation.Frontiers in molecular neuroscience · 2023Article
- The circadian clock mediates daily bursts of cell differentiation by periodically restricting cell-differentiation commitment.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish.International journal of molecular sciences · 2022Article
- The habenula clock influences response to a stressor.Neurobiology of stress · 2021Article
- Interconnections between circadian clocks and metabolism.The Journal of clinical investigation · 2021Review
- Correction: Single-cell in vivo imaging of cellular circadian oscillators in zebrafish.PLoS biology · 2021Article
- Circadian rhythms: influence on physiology, pharmacology, and therapeutic interventions.Journal of pharmacokinetics and pharmacodynamics · 2021Review
- Circadian regulation of muscle growth independent of locomotor activity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The circadian clock is a cell-autonomous time-keeping mechanism established gradually during embryonic development. Here, we generated a transgenic zebrafish line carrying a destabilized fluorescent protein driven by the promoter of a core clock gene, nr1d1, to report in vivo circadian rhythm at the single-cell level. By time-lapse imaging of this fish line and 3D reconstruction, we observed the sequential initiation of the reporter expression starting at photoreceptors in the pineal gland, then spreading to the cells in other brain regions at the single-cell level. Even within the pineal gland, we found heterogeneous onset of nr1d1 expression, in which each cell undergoes circadian oscillation superimposed over a cell type-specific developmental trajectory. Furthermore, we found that single-cell expression of nr1d1 showed synchronous circadian oscillation under a light-dark (LD) cycle. Remarkably, single-cell oscillations were dramatically dampened rather than desynchronized in animals raised under constant darkness, while the developmental trend still persists. It suggests that light exposure in early zebrafish embryos has significant effect on cellular circadian oscillations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.