Evidence map›Paper›PMID 32166810›Full record

ArticleVox sanguinis2020

Red-blood-cell manufacturing methods and storage solutions differentially induce pulmonary cell activation.

Mathijs R Wirtz, Ruqayyah J Almizraq, Nina C Weber, Philip J Norris, Suchitra Pandey, Philip C Spinella, Jennifer A Muszynski, Jason P Acker, Nicole P Juffermans

Open access · hybridAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Vox sanguinis, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 63% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Observational
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 3 countries.

Mathijs R Wirtz *Laboratory of Experimental Intensive Care and Anesthesiology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.ORCID https://orcid.org/0000-0002-1923-083X
Ruqayyah J Almizraq *Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
Nina C WeberLaboratory of Experimental Intensive Care and Anesthesiology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
Philip J NorrisBlood Systems Research Institute, San Francisco, CA, USA.
Suchitra PandeyDepartment of Laboratory Medicine, University of California, San Francisco, CA, USA.
Philip C SpinellaDepartment of Pediatrics, Division of Critical Care, Washington University in St Louis, St Louis, MO, USA.
Jennifer A MuszynskiDepartment of Pediatrics, Division of Critical Care Medicine, Nationwide Children's Hospital, Columbus, OH, USA.
Jason P AckerLaboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada.
Nicole P JuffermansLaboratory of Experimental Intensive Care and Anesthesiology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.
Amsterdam Neuroscience · NLUniversity of Alberta · CAUniversity of California, San Francisco · USAmsterdam University Medical Centers · NLNationwide Children's Hospital · USWashington University in St. Louis · US

Funding

Canadian Blood Services Intramural Grant program 2015IG-JA
6 · The paper itself

Abstract

BACKGROUND AND

objectivesRed-blood-cell (RBC) transfusion is associated with lung injury, which is further exacerbated by mechanical ventilation. Manufacturing methods of blood products differ globally and may play a role in the induction of pulmonary cell activation through alteration of the immunomodulatory property of the products. Here, the effect of different manufacturing methods on pulmonary cell activation was investigated in an in vitro model of mechanical ventilation. MATERIALS AND

methodsPulmonary type II cells were incubated with supernatant from fresh and old RBC products obtained via whole blood filtration (WBF), red cell filtration (RCF), apheresis-derived (AD) or whole blood-derived (WBD) methods. Lung cells were subjected to 25% stretch for 24 h. Controls were non-stretched or non-incubated cells.

resultsFresh but not old AD products and WBF products induce lung cell production of pro-inflammatory cytokines and chemokines, which was not observed with WBD or RCF products. Effects were associated with an increased amount of platelet-derived vesicles and an increased thrombin-generating capacity. Mechanical stretching of lung cells induced more severe cell injury compared to un-stretched controls, including alterations in the cytoskeleton, which was further augmented by incubation with AD products. In all read-out parameters, RCF products seemed to induce less injury compared to the other products.

conclusionsOur findings show that manufacturing methods of RBC products impact pulmonary cell activation, which may be mediated by the generation of vesicles in the product. We suggest RBC manufacturing method may be an important factor in understanding the association between RBC transfusion and lung injury.

Indexed as

Blood PreservationInflammationCytokinesErythrocytesErythrocyte TransfusionHumansLungRespiration, ArtificialThrombinCytokinesThrombinblood processingcytokine and chemokine productionextracellular vesiclesmechanical ventilationthrombin generation

Identifiers

PMID32166810
PMCPMC7497002
OpenAlexW3012011704

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.