Evidence map›Paper›PMID 32166385›Full record

ArticleJournal of molecular evolution2020

Evolutionary Diversity in the Intracellular Microsporidian Parasite Nosema sp. Infecting Wild Silkworm Revealed by IGS Nucleotide Sequence Diversity.

Wazid Hassan, Basavaraju Surendra Nath, Kangayam M Ponnuvel, Rakesh K Mishra, Appukuttan Nair R Pradeep

Abstract read
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In one paragraph

Article in Journal of molecular evolution, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Morphological characterization and genetic diversity of a new microsporidium,Frontiers in cellular and infection microbiology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Wazid HassanMolecular Pathology Division, Seribiotech Research Laboratory, Central Silk Board, CSB Campus, Carmelaram Post, Kodathi, Bangalore, Karnataka, 560035, India.
Basavaraju Surendra NathCentral Tasar Research and Training Institute, Central Silk Board, Ranchi, Jharkhand, 835303, India.
Kangayam M PonnuvelMolecular Pathology Division, Seribiotech Research Laboratory, Central Silk Board, CSB Campus, Carmelaram Post, Kodathi, Bangalore, Karnataka, 560035, India.
Rakesh K MishraProteomics Division, Seribiotech Research Laboratory, Central Silk Board, CSB Campus, Carmelaram Post, Kodathi, Bangalore, Karnataka, 560035, India.
Appukuttan Nair R PradeepProteomics Division, Seribiotech Research Laboratory, Central Silk Board, CSB Campus, Carmelaram Post, Kodathi, Bangalore, Karnataka, 560035, India. arpradeepnair@gmail.com.
Central Silk Technological Research Institute · INCentral Tasar Research & Training Institute · IN

Funding

Council of Scientific and Industrial Research, India Research Associateship
6 · The paper itself

Abstract

Intracellular microsporidian Nosema mylitta infects Indian wild silkworm Antheraea mylitta causing pebrine disease. Genetic structure and phylogeny of N. mylitta are analysed using nucleotide variability in 5S ribosomal DNA and intergenic spacer (IGS) sequence from 20 isolates collected from Southern, Northern and Central regions of Jharkhand State. Nucleotide diversity (π) and genetic differentiation Gst were highest in the Central isolates whereas lowest in the North. Among the isolates, absence of nucleotides, transitions and transversions were observed. Haplotyping showed nucleotide variability at 83 positions in IGS and 13 positions in 5S rDNA. Haplotype-based genetic differentiation was 0.96 to 0.97 whereas nucleotide sequence-based genetic differentiation was higher (Ks = 22.29) between Southern and Central isolates. Bottleneck analysis showed negative value for Tajima's D and other summary statistics revealing induction of loss of rare alleles and population explosion. From IGS, 17 ancestral sequences were inferred by Network algorithm. Core of nine closely related nodes having ancient nucleotides and peripheral nodes with highly divergent nucleotides were derived. Most diverged peripheral haplotype was Bero (H11) from the Central region whereas Deoghar (H3) of the Northern region diverged early. Phylogeny of N. mylitta grouped Southern and Northern isolates together revealed weak phylogenetic signal for these locations. Phylogeny of N. mylitta with Nosema sp. infecting other lepidopterans clustered N. mylitta isolates with N. antheraea and N. philosamiae of China indicating genetic similarity whereas other species were dissimilar showing diversity irrespective of country of origin.

Indexed as

BombyxNosemaAnimalsBase SequenceChinaDNA, IntergenicNucleotidesPhylogenyRNA, Ribosomal, 5SSequence Analysis, DNADNA, IntergenicNucleotidesRNA, Ribosomal, 5S5S ribosomal DNA sequenceAntheraea mylittaGenetic differentiationIntergenic spacer sequencePhylogenyTajima’s D

Identifiers

PMID32166385
OpenAlexW3010970628

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.