Evidence map›Paper›PMID 32165827›Full record

ArticleMolecular vision2020

MicroRNA-29b-3p inhibits cell proliferation and angiogenesis by targeting VEGFA and PDGFB in retinal microvascular endothelial cells.

Wenyi Tang, Jingli Guo, Ruiping Gu, Boya Lei, Xinyi Ding, Jun Ma, Gezhi Xu

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Molecular vision, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 32 citations in OpenAlex.

  1. Review
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  5. Current computer-aided drug design · 2025
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  7. Role of exosome-derived miRNAs in diabetic wound angiogenesis.Molecular and cellular biochemistry · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Wenyi TangDepartment of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Jingli GuoDepartment of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Ruiping GuDepartment of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Boya LeiDepartment of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Xinyi DingDepartment of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Jun MaShanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai, China.
Gezhi XuDepartment of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Eye & ENT Hospital of Fudan University · CNFudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: Excessive angiogenesis, also known as neovascularization, has considerable pathophysiologic roles in several retinal diseases, including retinopathy of prematurity, diabetic retinopathy, and exudative age-related macular degeneration. Accumulated evidence has revealed that miRNAs play important roles in endothelial cell dysfunction and angiogenesis. However, the role of microRNA-29b-3p (miR-29b-3p) in retinal angiogenesis is still unclear. Therefore, we investigated whether and how miR-29b-3p affects the function of retinal microvascular endothelial cells (RMECs). Methods: The overexpression and inhibition of miR-29b-3p were achieved by transfecting rat RMECs with an miR-29b-3p mimic and inhibitor, respectively. The proliferation, migration, and angiogenesis of RMECs were evaluated using a Cell Counting Kit-8 assay, Ki67 staining, western blotting (of proliferating cell nuclear antigen, cyclin A2, cyclin D1, and cyclin E1), wound healing test, and tube formation assay. The expression levels of vascular endothelial growth factor A (VEGFA) and platelet-derived growth factor B (PDGFB) were examined with quantitative real-time PCR and western blotting, respectively. Results: Overexpression of miR-29b-3p statistically significantly inhibited the function of RMECs in cell proliferation and angiogenesis, while inhibition of miR-29b-3p increased the proliferative and angiogenic activities of RMECs. Moreover, VEGFA and PDGFB, as the targets of miR-29b-3p, were statistically significantly downregulated by the miR-29b mimic, whereas the miR-29b-3p inhibitor had the opposite effects. Conclusions: miR-29b-3p negatively regulates RMEC proliferation and angiogenesis, at least partly by targeting VEGFA and PDGFB. These data may provide a potential therapeutic strategy for treating ocular neovascular diseases.

Indexed as

AnimalsCell ProliferationCells, CulturedCell SurvivalDown-RegulationEndothelial CellsMicroRNAsNeovascularization, PathologicPlatelet-Derived Growth FactorRatsRetinaTransfectionUp-RegulationVascular Endothelial Growth Factor AWound HealingMicroRNAsMIRN29 microRNA, ratPlatelet-Derived Growth FactorVascular Endothelial Growth Factor A

Identifiers

PMID32165827
PMCPMC7043642
OpenAlexW3012715625

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.