ArticleProceedings of the National Academy of Sciences of the United States of America2020
Molecular mechanisms of Evening Complex activity in
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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82 citing papers in PubMed, 161 citations in OpenAlex.
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- Early birds and night owls: natural variation of circadian traits in plants.Journal of experimental botany · 2026Review
- Epigenetic regulation under light and temperature fluctuations: chromatin remodeling and transcriptional memory in plants.Plant cell reports · 2026Review
- Epigenetic memory and systemic priming: an emerging framework for cold-resilient crops.Plant cell reports · 2026Review
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- Temperature compensation of the Arabidopsis circadian clock.Npj biological timing and sleep · 2025Review
- Emerging Mechanisms of Plant Responses to Abiotic Stress.Plants (Basel, Switzerland) · 2025Review
- The blue light receptor ZmFKF1a recruits ZmGI1 to the nucleus to accelerate shoot apex development and flowering in maize.The Plant cell · 2025Article
- Early Flowering 3 (ELF3) Inhibits Hypocotyl Phototropism in Light-GrownPlant direct · 2025Article
- Transcriptional activation and repression in the plant circadian clock: Revisiting core oscillator feedback loops and output pathways.Plant communications · 2025Review
- BBX24 and BBX25 antagonize the function of thermosensor ELF3 to promote PIF4-mediated thermomorphogenesis in Arabidopsis.Plant communications · 2025Article
- A multisensor high-temperature signaling framework for triggering daytime thermomorphogenesis in Arabidopsis.Nature communications · 2025Article
- Evolutionary Influences on Local Patterns of Genetic Relatedness.bioRxiv : the preprint server for biology · 2025Article
- Abundant clock proteins point to missing molecular regulation in the plant circadian clock.Molecular systems biology · 2025Article
- Stress resilience in plants: the complex interplay between heat stress memory and resetting.The New phytologist · 2025Review
- Combined Bulked Segregant Analysis-Sequencing and Transcriptome Analysis to Identify Candidate Genes Associated with Cold Stress inInternational journal of molecular sciences · 2025Article
- Whole-genome identification ofFrontiers in plant science · 2025Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Evening Complex (EC), composed of the DNA binding protein LUX ARRHYTHMO (LUX) and two additional proteins EARLY FLOWERING 3 (ELF3) and ELF4, is a transcriptional repressor complex and a core component of the plant circadian clock. In addition to maintaining oscillations in clock gene expression, the EC also participates in temperature and light entrainment, acting as an important environmental sensor and conveying this information to growth and developmental pathways. However, the molecular basis for EC DNA binding specificity and temperature-dependent activity were not known. Here, we solved the structure of the DNA binding domain of LUX in complex with DNA. Residues critical for high-affinity binding and direct base readout were determined and tested via site-directed mutagenesis in vitro and in vivo. Using extensive in vitro DNA binding assays of LUX alone and in complex with ELF3 and ELF4, we demonstrate that, while LUX alone binds DNA with high affinity, the LUX-ELF3 complex is a relatively poor binder of DNA. ELF4 restores binding to the complex. In vitro, the full EC is able to act as a direct thermosensor, with stronger DNA binding at 4 °C and weaker binding at 27 °C. In addition, an excess of ELF4 is able to restore EC binding even at 27 °C. Taken together, these data suggest that ELF4 is a key modulator of thermosensitive EC activity.
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