ArticleDevelopmental biology2020
Cell fusion is differentially regulated in zebrafish post-embryonic slow and fast muscle.
Article in Developmental biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Myomaker and Myomixer are required for craniofacial myoblast fusion in zebrafish.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Cell Fusion in Reprogramming and Regeneration.Advances in experimental medicine and biology · 2026Review
- Dynamic expression and differential requirement of the myocyte fusogen Myomixer during distinct myogenic episodes in the zebrafish.Biology open · 2025Article
- N-Myc downstream regulated gene 1b is a regulator of cell adhesion during early muscle development.bioRxiv : the preprint server for biology · 2025Article
- Ectopic expression of Myomaker and Myomixer in slow muscle cells induces slow muscle fusion and myofiber death.Journal of genetics and genomics = Yi chuan xue bao · 2024Article
- Myotube Guidance: Shaping up the Musculoskeletal System.Journal of developmental biology · 2024Review
- The Regulatory Role ofAnimals : an open access journal from MDPI · 2024Article
- Whole-body replacement of larval myofibers generates permanent adult myofibers in zebrafish.The EMBO journal · 2024Article
- Skeletal muscle regeneration after extensive cryoinjury of caudal myomeres in adult zebrafish.NPJ Regenerative medicine · 2024Article
- Molecular regulation of myocyte fusion.Current topics in developmental biology · 2024Review
- An improved Erk biosensor detects oscillatory Erk dynamics driven by mitotic erasure during early development.Developmental cell · 2023Article
- Surfaceome Profiling of Cell Lines and Patient-Derived Xenografts Confirm FGFR4, NCAM1, CD276, and Highlight AGRL2, JAM3, and L1CAM as Surface Targets for Rhabdomyosarcoma.International journal of molecular sciences · 2023Article
- Isolation, Culture, and Analysis of Zebrafish Myofibers and Associated Muscle Stem Cells to Explore Adult Skeletal Myogenesis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- CaThe FEBS journal · 2022Article
- Loss of Myomixer Results in Defective Myoblast Fusion, Impaired Muscle Growth, and Severe Myopathy in Zebrafish.Marine biotechnology (New York, N.Y.) · 2022Article
- The cellular architecture and molecular determinants of the zebrafish fusogenic synapse.Developmental cell · 2022Article
- Regulation of the myoblast fusion reaction for muscle development, regeneration, and adaptations.Experimental cell research · 2022Article
- Monocyte-Macrophage Lineage Cell Fusion.International journal of molecular sciences · 2022Review
- Impaired activity of the fusogenic micropeptide Myomixer causes myopathy resembling Carey-Fineman-Ziter syndrome.The Journal of clinical investigation · 2022Article
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Skeletal muscle fusion occurs during development, growth, and regeneration. To investigate how muscle fusion compares among different muscle cell types and developmental stages, we studied muscle cell fusion over time in wild-type, myomaker (mymk), and jam2a mutant zebrafish. Using live imaging, we show that embryonic myoblast elongation and fusion correlate tightly with slow muscle cell migration. In wild-type embryos, only fast muscle fibers are multinucleate, consistent with previous work showing that the cell fusion regulator gene mymk is specifically expressed throughout the embryonic fast muscle domain. However, by 3 weeks post-fertilization, slow muscle fibers also become multinucleate. At this late-larval stage, mymk is not expressed in muscle fibers, but is expressed in small cells near muscle fibers. Although previous work showed that both mymk and jam2a are required for embryonic fast muscle cell fusion, we observe that muscle force and function is almost normal in mymk and jam2a mutant embryos, despite the lack of fast muscle multinucleation. We show that genetic requirements change post-embryonically, with jam2a becoming much less important by late-larval stages and mymk now required for muscle fusion and growth in both fast and slow muscle cell types. Correspondingly, adult mymk mutants perform poorly in sprint and endurance tests compared to wild-type and jam2a mutants. We show that adult mymk mutant muscle contains small mononucleate myofibers with average myonuclear domain size equivalent to that in wild type adults. The mymk mutant fibers have decreased Laminin expression and increased numbers of Pax7-positive cells, suggesting that impaired fiber growth and active regeneration contribute to the muscle phenotype. Our findings identify several aspects of muscle fusion that change with time in slow and fast fibers as zebrafish develop beyond embryonic stages.
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Registered trials
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