Evidence map›Paper›PMID 32164546›Full record

ArticleBMC plant biology2020

The low-copy nuclear gene Agt1 as a novel DNA barcoding marker for Bromeliaceae.

Fabian Bratzel, Sascha Heller, Nadine Cyrannek, Juraj Paule, Elton M C Leme, Anna Loreth, Annika Nowotny, Markus Kiefer, Walter Till, Michael H J Barfuss and 3 more

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In one paragraph

Article in BMC plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Fabian BratzelDepartment of Botany and Molecular Evolution, Senckenberg Research Institute and Natural History Museum Frankfurt, Senckenberganlage 25, 60325, Frankfurt am Main, Germany. fabian.bratzel@senckenberg.de.
Sascha HellerInstitute for Ecology, Evolution and Diversity, Goethe University, Max-von-Laue-Straße 13, 60438, Frankfurt am Main, Germany.
Nadine CyrannekInstitute for Ecology, Evolution and Diversity, Goethe University, Max-von-Laue-Straße 13, 60438, Frankfurt am Main, Germany.
Juraj PauleDepartment of Botany and Molecular Evolution, Senckenberg Research Institute and Natural History Museum Frankfurt, Senckenberganlage 25, 60325, Frankfurt am Main, Germany.
Elton M C LemeMarie Selby Botanical Gardens, 811 South Palm Avenue, Sarasota, FL, 34236, USA.
Anna LorethCentre for Organismal Studies (COS) Heidelberg, Department for Biodiversity and Plant Systematics, University of Heidelberg, Im Neuenheimer Feld 345, 69120, Heidelberg, Germany.
Annika NowotnyCentre for Organismal Studies (COS) Heidelberg, Department for Biodiversity and Plant Systematics, University of Heidelberg, Im Neuenheimer Feld 345, 69120, Heidelberg, Germany.
Markus KieferCentre for Organismal Studies (COS) Heidelberg, Department for Biodiversity and Plant Systematics, University of Heidelberg, Im Neuenheimer Feld 345, 69120, Heidelberg, Germany.
Walter TillDepartment of Botany and Biodiversity Research, Faculty of Life Sciences, University of Vienna, Rennweg 14, 1030, Vienna, Austria.
Michael H J BarfussDepartment of Botany and Biodiversity Research, Faculty of Life Sciences, University of Vienna, Rennweg 14, 1030, Vienna, Austria.
Christian LexerDepartment of Botany and Biodiversity Research, Faculty of Life Sciences, University of Vienna, Rennweg 14, 1030, Vienna, Austria.
Marcus A KochCentre for Organismal Studies (COS) Heidelberg, Department for Biodiversity and Plant Systematics, University of Heidelberg, Im Neuenheimer Feld 345, 69120, Heidelberg, Germany.
Georg ZizkaDepartment of Botany and Molecular Evolution, Senckenberg Research Institute and Natural History Museum Frankfurt, Senckenberganlage 25, 60325, Frankfurt am Main, Germany.

Funding

Bundesministerium für Bildung und Forschung Evo-BoGa
6 · The paper itself

Abstract

backgroundThe angiosperm family Bromeliaceae comprises over 3.500 species characterized by exceptionally high morphological and ecological diversity, but a very low genetic variation. In many genera, plants are vegetatively very similar which makes determination of non flowering bromeliads difficult. This is particularly problematic with living collections where plants are often cultivated over decades without flowering. DNA barcoding is therefore a very promising approach to provide reliable and convenient assistance in species determination. However, the observed low genetic variation of canonical barcoding markers in bromeliads causes problems.

resultIn this study the low-copy nuclear gene Agt1 is identified as a novel DNA barcoding marker suitable for molecular identification of closely related bromeliad species. Combining a comparatively slowly evolving exon sequence with an adjacent, genetically highly variable intron, correctly matching MegaBLAST based species identification rate was found to be approximately double the highest rate yet reported for bromeliads using other barcode markers.

conclusionIn the present work, we characterize Agt1 as a novel plant DNA barcoding marker to be used for barcoding of bromeliads, a plant group with low genetic variation. Moreover, we provide a comprehensive marker sequence dataset for further use in the bromeliad research community.

Indexed as

DNA Barcoding, TaxonomicBromeliaceaeDNA, PlantPlant ProteinsDNA, PlantPlant ProteinsBromeliadsDNA barcodingLow-copy nuclear genePlant collections

Identifiers

PMID32164546
PMCPMC7068990

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.