ArticleInternational journal of molecular sciences2020
Primary Human Chondrocytes Affected by Cigarette Smoke-Therapeutic Challenges.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 26 citations in OpenAlex.
- Using Mendelian randomization analyses in osteoarthritis: state of art and future perspectives.Annals of medicine · 2025Pooled it
- Pooled it
- No association between alcohol consumption and hip osteoarthritis: a diverse national analysis of 87,585 adults from the "All of Us" research program.Clinical rheumatology · 2026Article
- Demographic Factors Affect the BMAC Proteome.Journal of proteome research · 2026Article
- Cigarette Smoke Extract Alters the Inflammatory Secretome of Osteoarthritis-Affected Synovium Increasing Joint Tissue Oxidative Stress in an Indirect Co-Culture Model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2026Article
- Longitudinal association of frailty levels with knee osteoarthritis in middle-aged and elderly chinese: a longitudinal cohort study.Scientific reports · 2026Article
- Cigarette and Electronic Cigarette Exposure in Osteoarthritis: Immune Dysregulation and Inflammatory Signaling Pathways.International journal of general medicine · 2026Article
- Article
- Preliminary study on the potential damage of cigarette smoke extract in 3D human chondrocyte culture.In vitro cellular & developmental biology. Animal · 2025Article
- Mechanistic insights into non-coding RNAs regulate autophagy in chondrocytes and their contribution to osteoarthritis.Frontiers in medicine · 2025Review
- Correlation between the ease of cage plates implantation and endplate Hounsfield unit value during ACDF: a retrospective study.BMC surgery · 2024Article
- Smoking and osteoimmunology: Understanding the interplay between bone metabolism and immune homeostasis.Journal of orthopaedic translation · 2024Review
- Association between sarcopenia and osteoarthritis among the US adults: a cross-sectional study.Scientific reports · 2024Article
- Effect of smoking on the redox status of knee osteoarthritis: A preliminary study.Experimental biology and medicine (Maywood, N.J.) · 2023Article
- The effects of cigarette smoking and nicotine on the therapeutic potential of mesenchymal stem cells.Histology and histopathology · 2022Review
- Adverse impact of smoking on the spine and spinal surgery.Surgical neurology international · 2021Review
- Maqui berry extract prevents cigarette smoke induced oxidative stress in human osteoblastsEXCLI journal · 2021Article
- Update on Novel Non-Operative Treatment for Osteoarthritis: Current Status and Future Trends.Frontiers in pharmacology · 2021Review
- Impact of cigarette smoke on the functions of mesenchymal stem cells.Cell transplantationReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Although several researchers have attested deleterious effects of smoking to the musculoskeletal system, the association between smoking and the onset of osteoarthritis (OA) remains unclear. Here, we investigate the effect of cigarette smoke extract (CSE) on primary human chondrocytes. The present study demonstrates that physiological concentrations of CSE (0.1%-10%) inhibit the viability, proliferation, and matrix formation of chondrocytes in a dose- and time-dependent manner. Significant amounts of free radicals were generated by 10% of CSE and led to cell death. A clinical dosage (4 mg/mL) of dexamethasone (Dex) showed toxic effects on chondrocytes, and the long-time treatment by lower doses (4-400 μg/mL) induced hypertrophic changes in the chondrocytes. To substitute Dex, diclofenac (Dic, 1 μg/mL) and acetaminophen (Ace, 10 μg/mL) were tested and did not worsen the metabolic activity of CSE-exposed chondrocytes. Hyaluronic acid (HA, 5 mg/mL) combined with Dic or Ace significantly inhibited the oxidative stress and enhanced the viability and matrix formation of CSE-exposed chondrocytes. This study shows for the first time that CSE mediates the disruption of cartilage through inducing cell death by increasing oxidative stress, and that this effect is fortified by Dex. The deleterious effects of CSE on chondrocytes could be reversed by treatment with HA combined with first-line analgesic/anti-inflammatory agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.