ArticlePloS one2020
Three-dimensional brain-on-chip model using human iPSC-derived GABAergic neurons and astrocytes: Butyrylcholinesterase post-treatment for acute malathion exposure.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Rapid Prototyping of Compartmentalized 3D Microfluidic Devices for Organotypic Cell Culture.Micromachines · 2026Article
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- Advancing Environmental ToxicologyEnvironment & health (Washington, D.C.) · 2024Review
- Effects of Amyloid Beta (Aβ) Oligomers on Blood-Brain Barrier Using a 3D Microfluidic Vasculature-on-a-Chip Model.Applied sciences (Basel, Switzerland) · 2024Article
- Patient-Derived Microphysiological Systems for Precision Medicine.Advanced healthcare materials · 2024Review
- The Diverse Roles of Reactive Astrocytes in the Pathogenesis of Amyotrophic Lateral Sclerosis.Brain sciences · 2024Review
- Review
- A Three-Dimensional Brain-on-a-Chip Using Human iPSC-Derived GABAergic Neurons and Astrocytes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Microphysiological systems to study tumor-stroma interactions in brain cancer.Brain research bulletin · 2021Review
- Article
- Protein-Based 3D Biofabrication of Biomaterials.Bioengineering (Basel, Switzerland) · 2021Review
- An Individual Patient's "Body" on Chips-How Organismoid Theory Can Translate Into Your Personal Precision Therapy Approach.Frontiers in medicine · 2021Article
- Three-DimensionalFrontiers in toxicology · 2021Review
- Chlorpyrifos Disrupts Acetylcholine Metabolism Across Model Blood-Brain Barrier.Frontiers in bioengineering and biotechnology · 2021Article
- Microphysiological Systems for Neurodegenerative Diseases in Central Nervous System.Micromachines · 2020Review
- Reconfigurable Microphysiological Systems for Modeling Innervation and Multitissue Interactions.Advanced biosystems · 2020Article
- Organ-on-a-chip engineering: Toward bridging the gap between lab and industry.Biomicrofluidics · 2020Review
- Induced pluripotent stem cell-based organ-on-a-chip as personalized drug screening tools: A focus on neurodegenerative disorders.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Organophosphates (OPs) induce acute and chronic neurotoxicity, primarily by inhibiting acetylcholinesterase (AChE) activity as well as by necrosis, and apoptosis. Butyrylcholinesterase (BuChE), an exogenous bioscavenger of OPs, can be used as a treatment for OP exposure. It is prerequisite to develop in vitro brain models that can study BuChE post-treatment for acute OP exposure. In this study, we developed a three-dimensional (3D) brain-on-chip platform with human induced pluripotent stem cell (iPSC)-derived neurons and astrocytes to simulate human brain behavior. The platform consists of two compartments: 1) a hydrogel embedded with human iPSC-derived GABAergic neurons and astrocytes and 2) a perfusion channel with dynamic medium flow. The brain tissue constructs were exposed to Malathion (MT) at various concentrations and then treated with BuChE after 20 minutes of MT exposure. Results show that the iPSC-derived neurons and astrocytes directly interacted and formed synapses in the 3D matrix, and that treatment with BuChE improved viability after MT exposure up to a concentration of 10-3 M. We conclude that the 3D brain-on-chip platform with human iPSC-derived brain cells is a suitable model to study the neurotoxicity of OP exposure and evaluate therapeutic compounds for treatment.
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Registered trials
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