ArticleAPL bioengineering2020
Development of 3D neuromuscular bioactuators.
Article in APL bioengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 70 citations in OpenAlex.
- Modular Tissue-Engineered Motor Units Featuring Spinal Motor Neurons Innervating Self-Assembled Myofiber Bundles.Macromolecular bioscience · 2026Article
- Limb-on-a-Chip: An All-Hydrogel Platform for Scalable and Reproducible Engineering of Functional Neuromuscular Tissues.Cell biomaterials · 2026Article
- Physiological and functional characterization for high-throughput optogenetic skeletal muscle exercise assays.Bioengineering & translational medicine · 2026Article
- Engineered Living Systems With Self-Organizing Neural Networks: From Anatomy to Behavior and Gene Expression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A forward-engineered, muscle-driven soft robotic swimmer.Science advances · 2025Article
- Tensile Forces and Nanofiber Alignment Influence Both Innervated and Non-Innervated Skeletal Myofiber Formation in Custom Mechanobioreactors.Biotechnology journal · 2025Article
- Basal Xenobot transcriptomics reveals changes and novel control modality in cells freed from organismal influence.Communications biology · 2025Article
- Advancing biohybrid robotics: Innovations in contraction models, control techniques, and applications.Biophysics reviews · 2025Review
- An automated platform for simultaneous, longitudinal analysis of engineered neuromuscular tissues for applications in neurotoxin potency testing.Current research in toxicology · 2025Article
- Ethics and responsibility in biohybrid robotics research.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Neuronal innervation regulates the secretion of neurotrophic myokines and exosomes from skeletal muscle.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Motor neurons and endothelial cells additively promote development and fusion of human iPSC-derived skeletal myocytes.Skeletal muscle · 2024Article
- Astrocyte-mediated Transduction of Muscle Fiber Contractions Synchronizes Hippocampal Neuronal Network Development.Neuroscience · 2023Article
- Optimization of Application-Driven Development ofTissue engineering. Part B, Reviews · 2022Review
- Multi-actuator light-controlled biological robots.APL bioengineering · 2022Article
- Will microfluidics enable functionally integrated biohybrid robots?Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Principles for the design of multicellular engineered living systems.APL bioengineering · 2022Article
- Current Progress in the Creation, Characterization, and Application of Human Stem Cell-derived in Vitro Neuromuscular Junction Models.Stem cell reviews and reports · 2022Review
- Bioengineered model of the human motor unit with physiologically functional neuromuscular junctions.Scientific reports · 2021Article
- Development of an objective index, neural activity score (NAS), reveals neural network ontogeny and treatment effects on microelectrode arrays.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neuronal control of skeletal muscle bioactuators represents a critical milestone toward the realization of future biohybrid machines that may generate complex motor patterns and autonomously navigate through their environment. Animals achieve these feats using neural networks that generate robust firing patterns and coordinate muscle activity through neuromuscular units. Here, we designed a versatile 3D neuron-muscle co-culture platform to serve as a test-bed for neuromuscular bioactuators. We used our platform in conjunction with microelectrode array electrophysiology to study the roles of synergistic interactions in the co-development of neural networks and muscle tissues. Our platform design enables co-culture of a neuronal cluster with up to four target muscle actuators, as well as quantification of muscle contraction forces. Using engineered muscle tissue targets, we first demonstrated the formation of functional neuromuscular bioactuators. We then investigated possible roles of long-range interactions in neuronal outgrowth patterns and observed preferential outgrowth toward muscles compared to the acellular matrix or fibroblasts, indicating muscle-specific chemotactic cues acting on motor neurons. Next, we showed that co-cultured muscle strips exhibited significantly higher spontaneous contractility as well as improved sarcomere assembly compared to muscles cultured alone. Finally, we performed microelectrode array measurements on neuronal cultures, which revealed that muscle-conditioned medium enhances overall neural firing rates and the emergence of synchronous bursting patterns. Overall, our study illustrates the significance of neuron-muscle cross talk for the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.