ArticleAPL bioengineering2020
Microfluidic platform for three-dimensional cell culture under spatiotemporal heterogeneity of oxygen tension.
Article in APL bioengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Dynamic and functional changes in vascular endothelial cell monolayers under combined hypoxic and inflammatory stimuli.Scientific reports · 2025Article
- pH-Dependent migratory behaviors of neutrophil-like cells in a microfluidic device with controllability of dissolved gas concentrations.Scientific reports · 2024Article
- Mimicking and analyzing the tumor microenvironment.Cell reports methods · 2024Review
- Hypoxia suppresses glucose-induced increases in collective cell migration in vascular endothelial cell monolayers.Scientific reports · 2024Article
- Biological characterization of breast cancer spheroid formed by fast fabrication method.In vitro models · 2024Article
- Microphysiological systems as models for immunologically 'cold' tumors.Frontiers in cell and developmental biology · 2024Review
- Improving tumor microenvironment assessment in chip systems through next-generation technology integration.Frontiers in bioengineering and biotechnology · 2024Review
- Microfluidic platform for the reproduction of hypoxic vascular microenvironments.Scientific reports · 2023Article
- The Applications and Challenges of the Development ofCellular and molecular bioengineering · 2023Review
- Middle-out methods for spatiotemporal tissue engineering of organoids.Nature reviews bioengineering · 2023Review
- Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis.Frontiers in microbiology · 2023Article
- Recapitulating Tumor Hypoxia in a Cleanroom-Free, Liquid-Pinning-Based Microfluidic Tumor Model.ACS biomaterials science & engineering · 2022Article
- Promising Strategies for the Development of Advanced In Vitro Models with High Predictive Power in Ischaemic Stroke Research.International journal of molecular sciences · 2022Review
- P21-activated kinase regulates oxygen-dependent migration of vascular endothelial cells in monolayers.Cell adhesion & migration · 2021Article
- Recent advances of integrated microfluidic suspension cell culture system.Engineering biology · 2021Review
- Article
- Oxygen Biosensors and Control in 3D Physiomimetic Experimental Models.Antioxidants (Basel, Switzerland) · 2021Review
- Diversity Models and Applications of 3D Breast Tumor-on-a-Chip.Micromachines · 2021Review
- Bone-on-a-chip: microfluidic technologies and microphysiologic models of bone tissue.Advanced functional materials · 2021Article
- 96-Well Oxygen Control Using a 3D-Printed Device.Analytical chemistry · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells in a tumor microenvironment are exposed to spatial and temporal variations in oxygen tension due to hyperproliferation and immature vascularization. Such spatiotemporal oxygen heterogeneity affects the behavior of cancer cells, leading to cancer growth and metastasis, and thus, it is essential to clarify the cellular responses of cancer cells to oxygen tension. Herein, we describe a new double-layer microfluidic device allowing the control of oxygen tension and the behavior of cancer cells under spatiotemporal oxygen heterogeneity. Two parallel gas channels were located above the media and gel channels to enhance gas exchange, and a gas-impermeable polycarbonate film was embedded in the device to prevent the diffusion of atmospheric oxygen. Variations in oxygen tension in the device with the experimental parameters and design variables were investigated computationally and validated by using oxygen-sensitive nanoparticles. The present device can generate a uniform hypoxic condition at oxygen levels down to 0.3% O
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.