ArticleBiology methods & protocols2017
Covalent modification of primers improves PCR amplification specificity and yield.
Article in Biology methods & protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- A novel Torque teno virus qPCR assay reveals longitudinal viral load variability among non-immunosuppressed subjects.EMBO molecular medicine · 2026Article
- Development of a Gold Nanoparticle-Assisted Multiplex Polymerase Chain Reaction Assay for Selected Hospital-Acquired Infections-Associated Bacterial and Fungal Pathogens.ChemistryOpen · 2026Article
- Molecular Evidence of ClonalFoods (Basel, Switzerland) · 2025Article
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Authors and funding
4 authors.
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Abstract
We report a method for covalent modification of primers that enhances the specificity of PCR and increases the yield of specific amplification products at the end of PCR. The introduction of thermally stable covalent modifications, such as alkyl groups to the exocyclic amines of deoxyadenosine or cytosine residues at the 3'-ends of primers results in enhanced specificity of reactions. This higher specificity can result in greater sensitivity of detection by reducing competition with non-productive reactions. The reduction in the amplification of unintended byproducts is most apparent when both primers are modified at their respective 3'-ends. The
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