ArticleBiology methods & protocols2017
CD-tagging-MS2: detecting allelic expression of endogenous mRNAs and their protein products in single cells.
Article in Biology methods & protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- Glucocorticoids enhance chemotherapy-driven stress granule assembly and impair granule dynamics, leading to cell death.Journal of cell science · 2022Article
- Imaging Organization of RNA Processing within the Nucleus.Cold Spring Harbor perspectives in biology · 2021Review
- Protocol for using TRIBE to study RNA-protein interactions and nuclear organization in mammalian cells.STAR protocols · 2021Article
- MS2-TRIBE Evaluates Both Protein-RNA Interactions and Nuclear Organization of Transcription by RNA Editing.iScience · 2020Article
- Cytoplasmic DNA can be detected by RNA fluorescence in situ hybridization.Nucleic acids research · 2019Article
- Uncoupling of nucleo-cytoplasmic RNA export and localization during stress.Nucleic acids research · 2019Article
- Visualizing nuclear RNAi activity in single living human cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Discriminating between the mRNA and protein outputs of each of the alleles of an endogenous gene in intact cells, is a difficult task. To examine endogenous transcripts originating from a specific allele, we applied Central Dogma tagging (CD-tagging), which is based on a tag insertion into an endogenous gene by creation of a new exon. Previously, CD-tagging was used to tag endogenous proteins. Here we developed a CD-tagging-MS2 approach in which two tags were inserted in tandem; a fluorescent protein tag in conjunction with the mRNA MS2 tag used for tagging mRNAs in cells. A cell clone library of CD-tagged-MS2 genes was generated, and protein and mRNA distributions were examined and characterized in single cells. Taking advantage of having one allele tagged, we demonstrate how the transcriptional activity of all alleles, tagged and untagged, can be identified using single molecule RNA fluorescence
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Registered trials
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