Evidence map›Paper›PMID 32161459›Full record

ArticleInternational journal of nanomedicine2020

Homing of Super Paramagnetic Iron Oxide Nanoparticles (SPIONs) Labeled Adipose-Derived Stem Cells by Magnetic Attraction in a Rat Model of Parkinson's Disease.

Ardeshir Moayeri, Marzieh Darvishi, Mansour Amraei

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of nanomedicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

  1. Pooled it
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  12. Application of magnetic nanoparticles in cell therapy.Stem cell research & therapy · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Ardeshir MoayeriDepartment of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.ORCID 0000-0001-6890-9390
Marzieh DarvishiDepartment of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Mansour AmraeiDepartment of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.ORCID 0000-0002-2520-4910
Medical University of Ilam · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionStem cell therapies for neurodegenerative diseases such as Parkinson's disease (PD) are intended to replace lost dopaminergic neurons. The basis of this treatment is to guide the migration of transplanted cells into the target tissue or injury site. The aim of this study is an evaluation of the homing of superparamagnetic iron oxide nanoparticles (SPIONs) labeled adipose-derived stem cells (ADSC) by an external magnetic field in a rat model of PD.

methodsADSCs were obtained from perinephric regions of male adult rats and cultured in a DMEM medium. ADSC markers were assessed by immunostaining with CD90, CD105, CD49d, and CD45. The SPION was coated using poly-L-lysine hydrobromide and transfection was determined in rat ADSC using the GFP reporter gene. For this in vivo study, rats with PD were divided into five groups: a positive control group, a control group with PD (lesion with 6-HD injection), and three treatment groups: the PD/ADSC group (PD transplant with ADSCs transfected by BrdU), PD/ADSC/SPION group (PD transplant with ADSCs labeled with SPION and transfected by GFP), and the PD/ADSC/SPION/EM group (PD transplant with ADSCs labeled with SPION and transfected by GFP induced with external magnet).

resultsADSCs were immunoreactive to fat markers CD90 (90.73±1.7), CD105 (87.4±2.9) and CD49d (79.6±2.6), with negative immunostaining at the hematopoietic stem cell marker (CD45: 1.4±0.4). The efficiency of cells with SPION/PLL was about 96% of ADSC. The highest number of GFP-positive cells was in the ADSC/SPION/EM group (54.5±1.3), which was significantly different from that in ADSC/SPION group (30.83±3 and P<0.01).

conclusionTransfection of ADSC by SPION/PLL is an appropriate protocol for cell therapy. External magnets can be used for the delivery and homing of transplanted stem cells in the target tissue.

Indexed as

Adipose TissueAnimalsBiomarkersDisease Models, AnimalFerrosoferric OxideGreen Fluorescent ProteinsMagnetite NanoparticlesMaleParkinson DiseaseRats, Sprague-DawleyStem CellsStem Cell TransplantationThy-1 AntigensTransfectionBiomarkersFerrosoferric OxideGreen Fluorescent ProteinsMagnetite NanoparticlesThy-1 Antigensadipose derived stem cellshomingstem cell therapysuper paramagnetic iron oxide nanoparticles

Identifiers

PMID32161459
PMCPMC7049746
OpenAlexW3008736509

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.