ArticleVeterinary research2020
Genetic modification of the protozoan Eimeria tenella using the CRISPR/Cas9 system.
Article in Veterinary research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Abnormal expression pattern of knock-in marker in Eimeria tenella using CRISPR/Cas9.Poultry science · 2026Article
- UnderstandingFrontiers in cellular and infection microbiology · 2026Review
- Eimeria tenella apical membrane 2 overexpression enhances pathogenicity and immunity in chickens.Parasites & vectors · 2025Article
- Comparative genomic analysis of fiveMicrobial genomics · 2025Article
- Genetic manipulation for the non-model protozoaniScience · 2025Review
- The F204S mutation in adrenodoxin oxidoreductase drives salinomycin resistance in Eimeria tenella.Veterinary research · 2024Article
- Oral delivery ofFrontiers in veterinary science · 2024Article
- Botanicals: A promising approach for controlling cecal coccidiosis in poultry.Frontiers in veterinary science · 2023Review
- Editorial: Insights in genome editing tools and mechanisms: 2022.Frontiers in genome editing · 2023Article
- Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities.Parasitology research · 2022Review
- Establishment of RecombinantVaccines · 2021Article
- Development of a CRISPR/Cas9 system in Entamoeba histolytica: proof of concept.International journal for parasitology · 2021Article
- Next-Generation Technologies and Systems Biology for the Design of Novel Vaccines Against Apicomplexan Parasites.Frontiers in veterinary science · 2021Review
- FnCas12a/crRNA-Mediated Genome Editing inFrontiers in genetics · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Eimeria tenella has emerged as valuable model organism for studying the biology and immunology of protozoan parasites with the establishment of the reverse genetic manipulation platform. In this report, we described the application of CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (endonuclease) system for efficient genetic editing in E. tenella, and showed that the CRISPR/Cas9 system mediates site-specific double-strand DNA breaks with a single guide RNA. Using this system, we successfully tagged the endogenous microneme protein 2 (EtMic2) by inserting the red fluorescent protein into the C-terminal of EtMic2. Our results extended the utility of the CRISPR/Cas9-mediated genetic modification system to E. tenella, and opened a new avenue for targeted investigation of gene functions in apicomplexan parasites.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.