ArticleBMC microbiology2020
Evaluation of real-time PCR targeting the lipL32 gene for diagnosis of Leptospira infection.
Article in BMC microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 3 of them syntheses that pooled it.
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Who cites it
33 citing papers in PubMed, 3 syntheses or guidelines pooled it, 61 citations in OpenAlex.
- Prevalence of leptospirosis among soldiers: A systematic review.PLoS neglected tropical diseases · 2025Pooled it
- Diagnosis of human leptospirosis: systematic review and meta-analysis of the diagnostic accuracy of the Leptospira microscopic agglutination test, PCR targeting Lfb1, and IgM ELISA to Leptospira fainei serovar Hurstbridge.BMC infectious diseases · 2024Pooled it
- Trends and advances inFrontiers in microbiology · 2024Pooled it
- Novel Target for Pathogenic Leptospira Detection via Colorimetric Loop-Mediated Isothermal Amplification (LAMP): Development, Validation, and Comparative Analysis.Journal of clinical laboratory analysis · 2026Article
- A Label-Free Graphene Quantum Dot-Based Fluorescent Aptasensor for Rapid and Sensitive Detection of Leptospira interrogans.Journal of fluorescence · 2026Article
- Genetic Diversity ofPathogens (Basel, Switzerland) · 2026Article
- Postflood Leptospirosis Outbreak Response Due toOpen forum infectious diseases · 2026Article
- Diagnostic Performance of Two Commercial qPCR Kits forTropical medicine and infectious disease · 2026Article
- Retrospective Molecular Detection and Characterization of PathogenicTropical medicine and infectious disease · 2026Article
- Leptospirosis in Southeast Asia: Investigating Seroprevalence, Transmission Patterns, and Diagnostic Challenges.Tropical medicine and infectious disease · 2026Review
- Laboratory Diagnosis of Leptospirosis.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Urine PCR testing as an effective method for early diagnosis of leptospirosis.Microbiology spectrum · 2025Article
- Prevalence of Salmonella, Cryptosporidium and Leptospira in Invasive Pond Slider (Trachemys scripta) in North-Western Italy.Veterinary medicine and science · 2025Article
- Leptospirosis.Nature reviews. Disease primers · 2025Review
- Uncovering latent infections in kidneys: A novel molecular approach for differentialCurrent research in microbial sciences · 2025Article
- Diagnostic Pathways ofPathogens (Basel, Switzerland) · 2024Article
- A retrospective study of animal leptospirosis in Kazakhstan.Journal of advanced veterinary and animal research · 2024Article
- Leptospirosis: a clinical and diagnostic challenge.BMJ case reports · 2024Article
- Production and characterization of immunoglobulin G anti-rLipL32 antibody as a biomarker for the diagnosis of leptospirosis.Veterinary world · 2024Article
- Case Report: Locally Acquired Leptospirosis in a Minnesota Boy and His Dog.The American journal of tropical medicine and hygiene · 2024Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 1 country.
Funding
Abstract
backgroundDifferent diagnostic methods have been used for the laboratory confirmation of leptospirosis. Molecular diagnostic techniques are not only faster and more sensitive than culture analysis, but can also detect a Leptospira infection before the appearance of antibodies. The aim of the present study was to analyze and compare two different PCR approaches applied to blood and urine specimens obtained from patients with clinical manifestations that were suggestive of leptospirosis. Furthermore, the results of these different PCR approaches were compared with the results of culture and serology analyses.
resultsA total of 400 samples (234 blood or 58.5% and 166 urine of 41.5%) from 310 Slovenian patients with clinical manifestations suggestive of leptospirosis were tested using conventional PCR assays targeting the rrs gene and RT-PCR targeting the lipL32 gene. Additionally, culture, serology and sequence analysis were performed for the majority of these samples. The PCR and RT-PCR results were concordant in 376 out of 400 of these samples (94.0%). Conventional PCR was positive for 27 out of 400 samples (6.8%) and RT-PCR was positive for 47 out of 400 samples (11.8%). Culture and microscopic agglutination tests supported these diagnoses.
conclusionsA comparison of the two PCR methods indicated that the RT-PCR targeting of the lipL32 gene was faster, more sensitive and more specific for the determination of Leptospira DNA in these clinical samples.
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