Evidence map›Paper›PMID 32160710›Full record

ArticleMolecular therapy. Nucleic acids2020

A Dual-Functioning 5'-PPP-NS1shRNA that Activates a RIG-I Antiviral Pathway and Suppresses Influenza NS1.

Neetu Singh, Priya Ranjan, Weiping Cao, Jenish Patel, Shivaprakash Gangappa, Bruce A Davidson, John M Sullivan, Paras N Prasad, Paul R Knight, Suryaprakash Sambhara

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular therapy. Nucleic acids, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Antiviral Approaches against Influenza Virus.Clinical microbiology reviews · 2023
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Neetu SinghInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA.
Priya RanjanInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA.
Weiping CaoInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA.
Jenish PatelInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA.
Shivaprakash GangappaInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA.
Bruce A DavidsonDepartment of Anesthesiology, State University of New York at Buffalo, Buffalo, NY 14203, USA; Department of Pathology and Anatomical Sciences, State University of New York at Buffalo, Buffalo, NY 14203, USA; Veterans Administration Western New York Healthcare System, Buffalo, NY 14215, USA.
John M SullivanDepartment of Ophthalmology, State University of New York at Buffalo, Buffalo, NY 14203, USA; Veterans Administration Western New York Healthcare System, Buffalo, NY 14215, USA.
Paras N PrasadDepartment of Chemistry, State University of New York at Buffalo, Buffalo, NY 14203, USA; Institute for Laser, Photonics, and Biophotonics, State University of New York at Buffalo, Buffalo, NY 14203, USA.
Paul R KnightDepartment of Anesthesiology, State University of New York at Buffalo, Buffalo, NY 14203, USA. Electronic address: pknight@buffalo.edu.
Suryaprakash SambharaInfluenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30329, USA. Electronic address: ssambhara@cdc.gov.
National Center for Immunization and Respiratory Diseases · USUniversity at Buffalo, State University of New York · US

Funding

Immunotherapy for acute lung injury secondary to influenzaR01HL151498 · NHLBI · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI KNIGHT III, PAUL R · 2020 to 2023
$1.7M
NHLBI NIH HHS R01 HL151498
6 · The paper itself

Abstract

Retinoic acid-inducible gene-I (RIG-I) is a cytosolic pathogen sensor that is crucial against a number of viral infections. Many viruses have evolved to inhibit pathogen sensors to suppress host innate immune responses. In the case of influenza, nonstructural protein 1 (NS1) suppresses RIG-I function, leading to viral replication, morbidity, and mortality. We show that silencing NS1 with in-vitro-transcribed 5'-triphosphate containing NS1 short hairpin RNA (shRNA) (5'-PPP-NS1shRNA), designed using the conserved region of a number of influenza viruses, not only prevented NS1 expression but also induced RIG-I activation and type I interferon (IFN) expression, resulting in an antiviral state leading to inhibition of influenza virus replication in vitro. In addition, administration of 5'-PPP-NS1shRNA in prophylactic and therapeutic settings resulted in significant inhibition of viral replication following viral challenge in vivo in mice with corresponding increases of RIG-I, IFN-β, and IFN-λ, as well as a decrease in NS1 expression.

Indexed as

5'PPP-RNAantiviralInfluenzaInterferonNS1RIG-IshRNA

Identifiers

PMID32160710
PMCPMC7049568
OpenAlexW3003865238

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.