Evidence map›Paper›PMID 32160650›Full record

ArticleJournal of cellular and molecular medicine2020

LncRNA ADAMTS9-AS2 suppresses the proliferation of gastric cancer cells and the tumorigenicity of cancer stem cells through regulating SPOP.

Feiran Wang, Chong Tang, Dong Xu, Yijie Tang, Yasu Jiang, Xuesong Gao, Junfei Xu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Feiran WangDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.ORCID 0000-0002-1790-7130
Chong TangDepartment of General Surgery, Affiliated Hospital 2 of Nantong University, Nantong, China.
Dong XuDepartment of General Surgery, Huai'an First People's Hospital, Huai'an, Jiangsu, China.
Yijie TangMedical College of Nantong University, Nantong, China.
Yasu JiangDepartment of General Surgery, Affiliated Hospital 2 of Nantong University, Nantong, China.
Xuesong GaoDepartment of General Surgery, Affiliated Hospital 2 of Nantong University, Nantong, China.
Junfei XuDepartment of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
Nantong University · CNHuaian First People’s Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nowadays, research on CSCs is still in an initial stage, and there are few studies reporting the successful isolation and identification of CSCs. In the present study, we attempted to isolate CSCs through cultivating the cell line MKN45 in defined serum-free medium and study the expression of stem cell markers or related proteins (Oct3/4, Sox2, Nanog and CD44) in CSCs. Moreover, immunofluorescence staining was performed to validate the stem cell markers of spheroid body-forming cells. Further experiments were used to evaluate the SPOP expression in tumorsphere cells. In addition, ADAMTS9-AS2 is a lncRNA that contributes to the genesis and development of many cancers, including gastric cancer (GC). We found ADAMTS9-AS2 functioned as an anti-oncogene and positively correlated with the expression of SPOP in GC tissues by combining bioinformatics analyses. Furthermore, we reported that ADAMTS9-AS2 regulated the expression of SPOP in GC cells and tumorsphere cells to inhibit GC progression. Together, our results demonstrated that SPOP and ADAMTS9-AS2 can be potential targets for GC treatment.

Indexed as

AnimalsCarcinogenicity TestsCell Line, TumorCell MovementCell ProliferationGene Expression Regulation, NeoplasticHeterograftsHumansMiceNeoplastic Stem CellsNuclear ProteinsRepressor ProteinsRNA, Long NoncodingSignal TransductionStomach NeoplasmslncRNA ADAMTS9-AS2, humanNuclear ProteinsRepressor ProteinsRNA, Long NoncodingSPOP protein, humanADAMTS9-AS2cancer stem cellsgastric cancerSpeckle-type POZ proteintumorsphere

Identifiers

PMID32160650
PMCPMC7176847
OpenAlexW3012443868

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.