Evidence map›Paper›PMID 32160533›Full record

ArticleCell reports2020

The GATA Transcription Factor Gaf1 Represses tRNAs, Inhibits Growth, and Extends Chronological Lifespan Downstream of Fission Yeast TORC1.

María Rodríguez-López, Suam Gonzalez, Olivia Hillson, Edward Tunnacliffe, Sandra Codlin, Victor A Tallada, Jürg Bähler, Charalampos Rallis

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 55 citations in OpenAlex.

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  15. Nuclear mRNA Export and Aging.International journal of molecular sciences · 2022
    Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Extension of chronological lifespan in Schizosaccharomyces pombe.Genes to cells : devoted to molecular & cellular mechanisms · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

María Rodríguez-LópezInstitute of Healthy Ageing and Department of Genetics, Evolution & Environment, University College London, London WC1E 6BT, UK.
Suam GonzalezSchool of Health, Sport and Bioscience, University of East London, Stratford Campus, London E14 4LZ, UK.
Olivia HillsonSchool of Health, Sport and Bioscience, University of East London, Stratford Campus, London E14 4LZ, UK.
Edward TunnacliffeInstitute of Healthy Ageing and Department of Genetics, Evolution & Environment, University College London, London WC1E 6BT, UK.
Sandra CodlinInstitute of Healthy Ageing and Department of Genetics, Evolution & Environment, University College London, London WC1E 6BT, UK.
Victor A TalladaCentro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide/CSIC, 41013 Sevilla, Spain.
Jürg BählerInstitute of Healthy Ageing and Department of Genetics, Evolution & Environment, University College London, London WC1E 6BT, UK. Electronic address: j.bahler@ucl.ac.uk.
Charalampos RallisInstitute of Healthy Ageing and Department of Genetics, Evolution & Environment, University College London, London WC1E 6BT, UK; School of Health, Sport and Bioscience, University of East London, Stratford Campus, London E14 4LZ, UK; School of Life Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK. Electronic address: c.rallis@essex.ac.uk.
University College London · GBUniversity of East London · GBCentro Andaluz de Biología del Desarrollo · ES

Funding

Biotechnology and Biological Sciences Research Council BB/R009597/1Wellcome TrustWellcome Trust 095598/Z/11/Z
6 · The paper itself

Abstract

Target of Rapamycin Complex 1 (TORC1) signaling promotes growth and aging. Inhibition of TORC1 leads to reduced protein translation, which promotes longevity. TORC1-dependent post-transcriptional regulation of protein translation has been well studied, while analogous transcriptional regulation is less understood. Here we screen fission yeast mutants for resistance to Torin1, which inhibits TORC1 and cell growth. Cells lacking the GATA factor Gaf1 (gaf1Δ) grow normally even in high doses of Torin1. The gaf1Δ mutation shortens the chronological lifespan of non-dividing cells and diminishes Torin1-mediated longevity. Expression profiling and genome-wide binding experiments show that upon TORC1 inhibition, Gaf1 directly upregulates genes for small-molecule metabolic pathways and indirectly represses genes for protein translation. Surprisingly, Gaf1 binds to and downregulates the tRNA genes, so it also functions as a transcription factor for RNA polymerase III. Thus, Gaf1 controls the transcription of both protein-coding and tRNA genes to inhibit translation and growth downstream of TORC1.

Indexed as

Gene Expression Regulation, FungalGenes, FungalMechanistic Target of Rapamycin Complex 1MutationNaphthyridinesOpen Reading FramesProtein BindingRNA, TransferSchizosaccharomycesSchizosaccharomyces pombe ProteinsTrans-ActivatorsTranscriptome1-(4-(4-propionylpiperazin-1-yl)-3-(trifluoromethyl)phenyl)-9-(quinolin-3-yl)benzo(h)(1,6)naphthyridin-2(1H)-oneGaf1 protein, S pombeMechanistic Target of Rapamycin Complex 1NaphthyridinesRNA, TransferSchizosaccharomyces pombe ProteinsTrans-Activatorsagingcell growthGATA6protein translationRNA polymerase IIIS. pombeTORtranscription factortRNAvacuole

Identifiers

PMID32160533
PMCPMC7068653
OpenAlexW3010918885

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.