ArticlePloS one2020
The relationship between environmental sources and the susceptibility of Acanthamoeba keratitis in the United Kingdom.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review.Memorias do Instituto Oswaldo Cruz · 2022Pooled it
- The potential of nanocomposites (patuletin-conjugated with gallic acid-coated zinc oxide) against free-living amoebae pathogens.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Morphological and Chemical Changes in the Trophozoites and Cysts of Acanthamoeba Castellanii Induced by Camellia Sinensis Extracts.Acta parasitologica · 2025Article
- Identification and Characterization of Acanthamoeba from Contact Lens Care Solutions and Hospital Environments in the Ophthalmology Wards and Operating Rooms in Southeastern Iran.Acta parasitologica · 2025Article
- Acanthamoeba keratitis: from pathophysiology to prevention, a contemporary clinical perspective.Medical hypothesis, discovery & innovation ophthalmology journal · 2025Review
- Epidemiology of and Genetic Factors Associated withPathogens (Basel, Switzerland) · 2024Review
- The role of naturally acquired intracellular Pseudomonas aeruginosa in the development of Acanthamoeba keratitis in an animal model.PLoS neglected tropical diseases · 2024Article
- A simple method for culturingClinical case reports · 2023Article
- Complete Recovery ofMicroorganisms · 2023Article
- Article
- Assessment of genotypes, endosymbionts and clinical characteristics of Acanthamoeba recovered from ocular infection.BMC infectious diseases · 2022Article
- [Current and future effects of climate change on ophthalmology].Die Ophthalmologie · 2022Review
- Article
- American Academy of Optometry Microbial Keratitis Think Tank.Optometry and vision science : official publication of the American Academy of Optometry · 2021Article
- Assessing the 3 pillars of housing for eye and vision health outcomes: A scoping review.Survey of ophthalmologyArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeTo determine whether Acanthamoeba keratitis (AK) patients have higher rates of Acanthamoeba and free-living amoeba (FLA) colonising domestic sinks than control contact lens (CL) wearers, and whether these isolates are genetically similar to the corneal isolates from their CL associated AK.
methods129 AK patients from Moorefield Eye Hospital, London and 64 control CL wearers from the Institute of Optometry were included in this study. The participants self-collected home kitchen and bathroom samples from tap-spouts, overflows and drains using an instructional kit. The samples were cultured by inoculating onto a non-nutrient agar plate seeded with Escherichia coli, incubated at 32°C and examined for amoebae by microscopy for up to 2 weeks. Partial sequences of mitochondrial cytochrome oxidase genes (coxA) of Acanthamoeba isolates from four AK patients were compared to Acanthamoeba isolated from the patient's home. The association between sampling sites was analysed with the chi-square test.
resultsA total of 513 samples from AK patients and 189 from CL controls were collected. The yield of FLA was significantly greater in patients' bathrooms (72.1%) than CL controls' bathrooms (53.4%) (p<0.05). Spouts (kitchen 6.7%, bathroom 11%) had the lowest rate of Acanthamoeba isolation compared to drains (kitchen 18.2%, bathroom 27.9%) and overflow (kitchen 39.1%, bathroom 25.9%) either in kitchens or bathrooms (p<0.05). There was no statistically significant difference between the average prevalence of Acanthamoeba in all three sample sites in kitchens (16.9%) compared to all three sample sites in bathrooms (21.5%) and no association for Acanthamoeba prevalence between AK patients and CL controls. All four corneal isolates had the same coxA sequence as at least one domestic water isolate from the patients' sink of the kitchen and the bathroom.
conclusionThe prevalence of Acanthamoeba and FLA was high in UK homes. FLA colonisation was higher in AK patients compared to controls but the prevalence of Acanthamoeba between AK patients and CL controls domestic sinks was similar. This study confirms that domestic water isolates are probably the source of AK infection. Advice about avoiding water contact when using CL's should be mandatory.
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