ArticlePloS one2020
Transcriptome analysis of the plant pathogen Sclerotinia sclerotiorum interaction with resistant and susceptible canola (Brassica napus) lines.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed.
- Article
- Turning Susceptibility into Strength: A New Era of Durable Resistance in Plants Through Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Genome-wide identification and characterization of transcription factors involved in defense responses against Sclerotinia sclerotiorum in Brassica juncea.Scientific reports · 2025Article
- Nano-chitosan-coated, green-synthesized selenium nanoparticles as a novel antifungal agent against Sclerotinia sclerotiorum: in vitro study.Scientific reports · 2025Article
- White Mold: A Global Threat to Crops and Key Strategies for Its Sustainable Management.Microorganisms · 2024Review
- An improved bacterial mRNA enrichment strategy in dual RNA sequencing to unveil the dynamics of plant-bacterial interactions.Plant methods · 2024Article
- Comparative transcriptome analysis in two contrasting genotypes for Sclerotinia sclerotiorum resistance in sunflower.PloS one · 2024Article
- Article
- Review
- Brassicaceae Fungi and Chromista Diseases: Molecular Detection and Host–Plant InteractionPlants (Basel, Switzerland) · 2023Review
- First genome-scale insights into the virulence of the snow mold causal fungus Microdochium nivale.IMA fungus · 2023Article
- Comparative transcriptome profiling reveals differential defense responses amongFrontiers in plant science · 2023Article
- Enhanced Resistance toInternational journal of molecular sciences · 2022Article
- Transcriptome Analysis of Wheat-Plants (Basel, Switzerland) · 2022Article
- Characterization of Transcriptional Responses to Genomovirus Infection of the White Mold Fungus,Viruses · 2022Article
- Identification of genetic loci in lettuce mediating quantitative resistance to fungal pathogens.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Genetic mapping and genomic prediction of sclerotinia stem rot resistance to rapeseed/canola (Brassica napus L.) at seedling stage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Genome-wide identification of the pectin methylesterase inhibitor genes inFrontiers in plant science · 2022Article
- Comparative Transcriptome Analysis of Fungal PathogenFrontiers in microbiology · 2022Article
- A Secondary Metabolism Pathway Involved in the Production of a Putative Toxin Is Expressed at Early Stage ofFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sclerotinia stem rot is an economically important disease of canola (Brassica napus) and is caused by the fungal pathogen Sclerotinia sclerotiorum. This study evaluated the differential gene expression patterns of S. sclerotiorum during disease development on two canola lines differing in susceptibility to this pathogen. Sequencing of the mRNA libraries derived from inoculated petioles and mycelium grown on liquid medium generated approximately 164 million Illumina reads, including 95 million 75-bp-single reads, and 69 million 50-bp-paired end reads. Overall, 36% of the quality filter-passed reads were mapped to the S. sclerotiorum reference genome. On the susceptible line, 1301 and 1214 S. sclerotiorum genes were differentially expressed at early (8-16 hours post inoculation (hpi)) and late (24-48 hpi) infection stages, respectively, while on the resistant line, 1311 and 1335 genes were differentially expressed at these stages, respectively. Gene ontology (GO) categories associated with cell wall degradation, detoxification of host metabolites, peroxisome related activities like fatty acid ß-oxidation, glyoxylate cycle, oxidoreductase activity were significantly enriched in the up-regulated gene sets on both susceptible and resistant lines. Quantitative RT-PCR of six selected DEGs further validated the RNA-seq differential gene expression analysis. The regulation of effector genes involved in host defense suppression or evasion during the early infection stage, and the expression of effectors involved in host cell death in the late stage of infection provide supporting evidence for a two-phase infection model involving a brief biotrophic phase during early stages of infection. The findings from this study emphasize the role of peroxisome related pathways along with cell wall degradation and detoxification of host metabolites as the key mechanisms underlying pathogenesis of S. sclerotiorum on B. napus.
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