Evidence map›Paper›PMID 32160192›Full record

ArticlePloS one2020

Pragmatic selection of larval mosquito diets for insectary rearing of Anopheles gambiae and Aedes aegypti.

Mark Q Benedict, Catherine M Hunt, Michael G Vella, Kasandra M Gonzalez, Ellen M Dotson, C Matilda Collins

Abstract readComparative Study
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Scent and adhesion drive insect dispersal of environmental yeast.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. [Effects of larval feeding amount on development and deltamethrin resistance inNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2025
    Article
  7. Article
  8. Batch RearingCold Spring Harbor protocols · 2023
    Article
  9. Article
  10. Review
  11. Review
  12. Article
  13. Next-generation gene drive for population modification of the malaria vector mosquito,Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mark Q BenedictDivision of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Entomology Branch, Atlanta, Georgia, United States of America.ORCID 0000-0001-5494-0522
Catherine M HuntDivision of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Entomology Branch, Atlanta, Georgia, United States of America.ORCID 0000-0002-4788-0207
Michael G VellaFrontier Scientific Services, Newark, Delaware, United States of America.
Kasandra M GonzalezFrontier Scientific Services, Newark, Delaware, United States of America.
Ellen M DotsonDivision of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Entomology Branch, Atlanta, Georgia, United States of America.
C Matilda CollinsCentre for Environmental Policy, Imperial College London, London, United Kingdom.ORCID 0000-0003-0966-5343

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Larval mosquitoes are aquatic omnivorous scavengers which scrape food from submerged surfaces and collect suspended food particles with their mouth brushes. The composition of diets that have been used in insectaries varies widely though necessarily provides sufficient nutrition to allow colonies to be maintained. Issues such as cost, availability and experience influence which diet is selected. One component of larval diets, essential fatty acids, appears to be necessary for normal flight though deficiencies may not be evident in laboratory cages and are likely more important when mosquitoes are reared for release into the field in e.g. mark-release-recapture and genetic control activities. In this study, four diets were compared for rearing Anopheles gambiae and Aedes aegypti, all of which provide these essential fatty acids. Two diets were custom formulations specifically designed for mosquitoes (Damiens) and two were commercially available fish foods: Doctors Foster and Smith Koi Staple Diet and TetraMin Plus Flakes. Development rate, survival, dry weight and adult longevity of mosquitoes reared with these four diets were measured. The method of presentation of one diet, Koi pellets, was additionally fed in two forms, pellets or a slurry, to determine any effect of food presentation on survival and development rate. While various criteria might be selected to choose 'the best' food, the readily-available Koi pellets resulted in development rates and adult longevity equal to the other diets, high survival to the adult stage and, additionally, this is available at low cost.

Indexed as

AedesAnimalsAnophelesBody WeightDietFatty Acids, EssentialFemaleFoodHousing, AnimalLarvaLongevityMaleSurvival RateTemperatureWaterFatty Acids, EssentialWater

Identifiers

PMID32160192
PMCPMC7065774

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.