Evidence map›Paper›PMID 32160106›Full record

ArticleRNA biology2020

Identification of alternatively spliced gene isoforms and novel noncoding RNAs by single-molecule long-read sequencing in

Zhikang Hu, Tao Lyu, Chao Yan, Yupeng Wang, Ning Ye, Zhengqi Fan, Xinlei Li, Jiyuan Li, Hengfu Yin

Open access · bronzeAbstract read
In one paragraph

Article in RNA biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 17 citations in OpenAlex.

  1. Genome-Wide Analysis of MADS-Box Gene Family RevealsInternational journal of molecular sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Zhikang HuState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry , Hangzhou, Zhejiang, China.
Tao LyuState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry , Hangzhou, Zhejiang, China.
Chao YanState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry , Hangzhou, Zhejiang, China.
Yupeng WangState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry , Hangzhou, Zhejiang, China.
Ning YeCollege of Information Science and Technology, Nanjing Forestry University , Nanjing, China.ORCID 0000-0001-7249-8352
Zhengqi FanKey Laboratory of Forest Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry , Hangzhou, Zhejiang, China.
Xinlei LiKey Laboratory of Forest Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry , Hangzhou, Zhejiang, China.
Jiyuan LiKey Laboratory of Forest Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry , Hangzhou, Zhejiang, China.
Hengfu YinState Key Laboratory of Tree Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry , Hangzhou, Zhejiang, China.ORCID 0000-0002-0720-5311
Chinese Academy of Forestry · CNNanjing Forestry University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Direct single-molecule sequencing of full-length transcripts allows efficient identification of gene isoforms, which is apt to alternative splicing (AS), polyadenylation, and long non-coding RNA analyses. However, the identification of gene isoforms and long non-coding RNAs with novel regulatory functions remains challenging, especially for species without a reference genome. Here, we present a comprehensive analysis of a combined long-read and short-read transcriptome sequencing in

Indexed as

Alternative SplicingGene Expression Regulation, PlantHigh-Throughput Nucleotide SequencingSingle Molecule ImagingCamelliaComputational BiologyGene Expression ProfilingGenome, PlantMolecular Sequence AnnotationPhenotypePolyadenylationRNA IsoformsRNA, UntranslatedTranscriptomeRNA IsoformsRNA, UntranslatedAlternative splicingCamellialipoxygenasephased small interfering RNApolyadenylationsingle-molecule sequencing

Identifiers

PMID32160106
PMCPMC7549672
OpenAlexW3010775936

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.