Evidence map›Paper›PMID 32158910›Full record

ArticleSmall methods2018

Advanced 3D Analysis and Optimization of Single-Molecule FISH in

Akiko Noma, Carlas S Smith, Maximiliaan Huisman, Robert M Martin, Melissa J Moore, David Grunwald

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Akiko NomaRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Carlas S SmithRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Maximiliaan HuismanRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Robert M MartinInstituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, 1600-276 Lisboa, Portugal.
Melissa J MooreRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA.
David GrunwaldRNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA 01605, USA.
University of Massachusetts Chan Medical School · USUniversity of Lisbon · PT

Funding

Canonical and non-canonical nuclear HIV-RNA gene export in living cellsR01GM123541 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI GRUNWALD, DAVID · 2016 to 2019
$1.3M
Visualizing local and global chromatin architecture, and gene expression in the individual cell by structural (SUSHI) and temporal (3D-SMRT) single molecule imagingU01DA047733 · NIDA · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI GRUNWALD, DAVID · 2018 to 2019
$1.0M
Visualizing local and global chromatin architecture, and gene expression in the individual cell by structural (SUSHI) and temporal (3D-SMRT) single molecule imagingU01EB021238 · NIBIB · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI GRUNWALD, DAVID · 2015 to 2017
$990k
NIBIB NIH HHS U01 EB021238NIDA NIH HHS U01 DA047733NIGMS NIH HHS R01 GM123541
6 · The paper itself

Abstract

Single-molecule fluorescence in situ hybridization (smFISH) provides direct access to the spatial relationship between nucleic acids and specific subcellular locations. The ability to precisely localize a messenger RNA can reveal key information about its regulation. Although smFISH is well established in cell culture or thin sections, the utility of smFISH is hindered in thick tissue sections due to the poor probe penetration of fixed tissue, the inaccessibility of target mRNAs for probe hybridization, high background fluorescence, spherical aberration along the optical axis, and the lack of methods for image segmentation of organelles. Studying mRNA localization in 50 μm thick

Indexed as

image processingsegmentationsmFISH

Identifiers

PMID32158910
PMCPMC7063683
OpenAlexW2807437878

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.