ArticlePlant reproduction2020
Loss of function of Oryza sativa Argonaute 18 induces male sterility and reduction in phased small RNAs.
Article in Plant reproduction, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Overexpression of OsAGO18 Promotes Early Seedling Development and Root Elongation in Rice.Plants (Basel, Switzerland) · 2026Article
- Reproductive phasiRNAs are the piRNAs of plants.Trends in genetics : TIG · 2026Review
- Epigenetic control of seed development and dormancy in cereals.Plant signaling & behavior · 2025Review
- Loss of function of chromatin remodeler OsCLSY4 leads to RdDM-mediated mis-expression of endosperm-specific genes affecting grain qualities.PLoS genetics · 2025Article
- Spatiotemporal regulation of target mRNA cleavage by 21-nt phasiRNAs in maize anthers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Major components of RNAi gene families inHeliyon · 2024Article
- Utilizing Short Interspersed Nuclear Element as a Genetic Marker for Pre-Harvest Sprouting in Wheat.Plants (Basel, Switzerland) · 2024Article
- Upstream regulator of genomic imprinting in rice endosperm is a small RNA-associated chromatin remodeler.Nature communications · 2024Article
- Premeiotic 24-nt phasiRNAs are present in theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Premeiotic 24-nt phasiRNAs are present in thebioRxiv : the preprint server for biology · 2024Article
- Phylogenetic analyses of seven protein families refine the evolution of small RNA pathways in green plants.Plant physiology · 2023Article
- Degradome comparison between wild and cultivated rice identifies differential targeting by miRNAs.BMC genomics · 2022Article
- Mechanisms of MicroRNA Biogenesis and Stability Control in Plants.Frontiers in plant science · 2022Review
- Homology-based identification of candidate genes for male sterility editing in upland cotton (Frontiers in plant science · 2022Review
- Essential role of γ-clade RNA-dependent RNA polymerases in rice development and yield-related traits is linked to their atypical polymerase activities regulating specific genomic regions.The New phytologist · 2021Article
- Male sterile 28 encodes an ARGONAUTE family protein essential for male fertility in maize.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Article
- Transgenerational conditioned male fertility of HD-ZIP IV transcription factor mutant ocl4: impact on 21-nt phasiRNA accumulation in pre-meiotic maize anthers.Plant reproduction · 2021Article
- Rice-specific Argonaute 17 controls reproductive growth and yield-associated phenotypes.Plant molecular biology · 2021Article
- A transposon surveillance mechanism that safeguards plant male fertility during stress.Nature plants · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageIn this manuscript, we show that Oryza sativa indica Argonaute protein AGO18 is required for male gametophyte development likely to through a small RNA-mediated mechanism. Monocots have evolved unique gene silencing pathways due to the presence of unique members of Dicer-like and Argonaute (AGO) family members. Among the monocot AGO homologs, AGO18 occupies a unique position. Previous reports have implicated this protein in viral resistance as well as in gametogenesis, likely through its competition with AGO1 clade members for micro(mi)RNAs and other small (s)RNAs. Although expression of rice AGO18 in specific stages of male gametogenesis has been documented, its major functions in plant development remain poorly understood. Here, we show that Oryza sativa indica AGO18 is involved in male gametophyte development. Knockdown (KD) of AGO18 in transgenic rice lines resulted in stunted plants that are male sterile, whereas their carpels were functional. Transcriptome analysis revealed downregulation of several pollen development-associated genes in KD lines. sRNA sequencing in vegetative and reproductive tissues of KD lines indicated reduction of miRNAs and phased secondary sRNAs implicated in male gametophyte development. Our results indicate a distinct role for rice AGO18 in male fertility.
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