ArticleScientific reports2020
Rapid detection of CITES-listed shark fin species by loop-mediated isothermal amplification assay with potential for field use.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Integrating portable qPCR and image recognition to combat illegal trade in sharks and rays.Scientific reports · 2025Article
- Rapid DNA/eDNA-Based ID Tools for Improved Chondrichthyan Monitoring and Management.Molecular ecology resources · 2025Review
- In Troubled Waters: Applying DNA Barcoding to Monitor Singapore's Shark Fin Trade.Ecology and evolution · 2025Article
- A universal DNA microarray for rapid fish species authentication.Food chemistry. Molecular sciences · 2025Article
- Small sharks, big problems: DNA analysis of small fins reveals trade regulation gaps and burgeoning trade in juvenile sharks.Science advances · 2024Article
- Environmental DNA as a tool to reconstruct catch composition for longline fisheries vessels.Scientific reports · 2024Article
- Article
- DNA barcodes are ineffective for species identification ofBiodiversity data journal · 2024Article
- Genetic identification of three CITES-listed sharks using a paper-based Lab-on-a-Chip (LOC).PloS one · 2024Article
- Current State of Milk, Dairy Products, Meat and Meat Products, Eggs, Fish and Fishery Products Authentication and Chemometrics.Foods (Basel, Switzerland) · 2023Review
- Identification of Fish Species and Targeted Genetic Modifications Based on DNA Analysis: State of the Art.Foods (Basel, Switzerland) · 2023Review
- A Fast and Simple DNA Mini-barcoding and RPA Assay Coupled with Lateral Flow Assay for Fresh and Canned Mackerel Authentication.Food analytical methods · 2023Article
- Seeing through sedimented waters: environmental DNA reduces the phantom diversity of sharks and rays in turbid marine habitats.BMC ecology and evolution · 2021Article
- Assessing the reliability of medicinal Dendrobium sequences in GenBank for botanical species identification.Scientific reports · 2021Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shark fin is a delicacy in many Asian countries. Overexploitation of sharks for shark fin trading has led to a drastic reduction in shark population. To monitor international trade of shark fin products and protect the endangered species from further population decline, we present rapid, user-friendly and sensitive diagnostic loop-mediated isothermal amplification (LAMP) and effective polymerase chain reaction (PCR) assays for all twelve CITES-listed shark species. Species-specific LAMP and PCR primers were designed based on cytochrome oxidase I (COI) and NADH2 regions. Our LAMP and PCR assays have been tested on 291 samples from 93 shark and related species. Target shark species could be differentiated from non-target species within three hours from DNA extraction to LAMP assay. The LAMP assay reported here is a simple and robust solution for on-site detection of CITES-listed shark species with shark fin products.
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