ArticleNature communications2020
Caliciviral protein-based artificial translational activator for mammalian gene circuits with RNA-only delivery.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Post-transcriptional modular synthetic receptors.Nature chemical biology · 2025Article
- Optogenetic Tools for Regulating RNA Metabolism and Functions.Chembiochem : a European journal of chemical biology · 2024Review
- Post-Transcriptional Modular Synthetic Receptors.bioRxiv : the preprint server for biology · 2024Article
- Development of a new caged intein for multi-input conditional translation of synthetic mRNA.Scientific reports · 2024Article
- Engineered poly(A)-surrogates for translational regulation and therapeutic biocomputation in mammalian cells.Cell research · 2024Article
- A Genetic Circuit Design for Targeted Viral RNA Degradation.Bioengineering (Basel, Switzerland) · 2023Article
- Dual Input-Controlled Synthetic mRNA Circuit for Bidirectional Protein Expression Regulation.ACS synthetic biology · 2023Article
- Programmable mammalian translational modulators by CRISPR-associated proteins.Nature communications · 2023Article
- Synthetic circular RNA switches and circuits that control protein expression in mammalian cells.Nucleic acids research · 2023Article
- Sensing intracellular signatures with synthetic mRNAs.RNA biology · 2023Review
- A protocol to construct RNA-protein devices for photochemical translational regulation of synthetic mRNAs in mammalian cells.STAR protocols · 2022Article
- Protein-Based Systems for Translational Regulation of Synthetic mRNAs in Mammalian Cells.Life (Basel, Switzerland) · 2021Review
- Exosome-mediated delivery of inflammation-responsiveTheranostics · 2021Article
- Rational Design of Aptamer-Tagged tRNAs.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic RNA-based gene circuits enable sophisticated gene regulation without the risk of insertional mutagenesis. While various RNA binding proteins have been used for translational repression in gene circuits, the direct translational activation of synthetic mRNAs has not been achieved. Here we develop Caliciviral VPg-based Translational activator (CaVT), which activates the translation of synthetic mRNAs without the canonical 5'-cap. The level of translation can be modulated by changing the locations, sequences, and modified nucleosides of CaVT-binding motifs in the target mRNAs, enabling the simultaneous translational activation and repression of different mRNAs with RNA-only delivery. We demonstrate the efficient regulation of apoptosis and genome editing by tuning translation levels with CaVT. In addition, we design programmable CaVT that responds to endogenous microRNAs or small molecules, achieving both cell-state-specific and conditional translational activation from synthetic mRNAs. CaVT will become an important tool in synthetic biology for both biological studies and future therapeutic applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.