ArticlemSystems2020
Complementary Metagenomic Approaches Improve Reconstruction of Microbial Diversity in a Forest Soil.
Article in mSystems, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Soil Microeukaryote Diversity in the Crop Fields of Panama.Microorganisms · 2026Article
- Comparative metagenomic assessment of Illumina-compatible library preparation methods, short-read lengths, and PacBio HiFi sequencing reveals differences in microbial and functional diversity recovery from a complex environmental sample.Microbiology spectrum · 2026Article
- Taxonomic and Functional Comparative Metagenomics of Peruvian Salterns: Insights into Microbial Communities and Aminotransferase Potential.Microorganisms · 2026Article
- Cultivation-independent high-quality microbial genome reconstruction from environmental samples with midi-metagenomics.Genome research · 2026Article
- Large-scale estimation of bacterial and archaeal DNA prevalence in metagenomes reveals biome-specific patterns.mSystems · 2026Article
- Global metagenomics reveals plastid diversity and unexplored algal lineages.Nature communications · 2026Article
- Microbial niches at the One Health interface: linking environmental factors, biomes, and disease dynamics.Frontiers in microbiology · 2026Review
- Genome-resolved long-read sequencing expands known microbial diversity across terrestrial habitats.Nature microbiology · 2025Article
- How genomics can help unravel the evolution of endophytic fungi.World journal of microbiology & biotechnology · 2025Review
- Recovery of 679 metagenome-assembled genomes from different soil depths along a precipitation gradient.Scientific data · 2025Article
- Metagenomic insights of microbial functions under conventional and conservation agriculture.World journal of microbiology & biotechnology · 2025Article
- GenomeOcean: An Efficient Genome Foundation Model Trained on Large-Scale Metagenomic Assemblies.bioRxiv : the preprint server for biology · 2025Article
- Single-cell genomics of single soil aggregates: methodological assessment and potential implications with a focus on nitrogen metabolism.Frontiers in microbiology · 2025Article
- Trait biases in microbial reference genomes.Scientific data · 2023Article
- Combining Flow Cytometry and Metagenomics Improves Recovery of Metagenome-Assembled Genomes in a Cell Culture from Activated Sludge.Microorganisms · 2023Article
- Article
- Cultivation-independent genomes greatly expand taxonomic-profiling capabilities of mOTUs across various environments.Microbiome · 2022Article
- Past and present giant viruses diversity explored through permafrost metagenomics.Nature communications · 2022Article
- A widely distributed genus of soil Acidobacteria genomically enriched in biosynthetic gene clusters.ISME communications · 2022Article
- The life cycle-dependent transcriptional profile of the obligate intracellular amoeba symbiont Amoebophilus asiaticus.FEMS microbiology ecology · 2022Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soil ecosystems harbor diverse microorganisms and yet remain only partially characterized as neither single-cell sequencing nor whole-community sequencing offers a complete picture of these complex communities. Thus, the genetic and metabolic potential of this "uncultivated majority" remains underexplored. To address these challenges, we applied a pooled-cell-sorting-based mini-metagenomics approach and compared the results to bulk metagenomics. Informatic binning of these data produced 200 mini-metagenome assembled genomes (sorted-MAGs) and 29 bulk metagenome assembled genomes (MAGs). The sorted and bulk MAGs increased the known phylogenetic diversity of soil taxa by 7.2% with respect to the Joint Genome Institute IMG/M database and showed clade-specific sequence recruitment patterns across diverse terrestrial soil metagenomes. Additionally, sorted-MAGs expanded the rare biosphere not captured through MAGs from bulk sequences, exemplified through phylogenetic and functional analyses of members of the phylum
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