ArticleEnvironmental microbiology2020
Integrative omics analysis of Pseudomonas aeruginosa virus PA5oct highlights the molecular complexity of jumbo phages.
Article in Environmental microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 50 citations in OpenAlex.
- The PhageExpressionAtlas reveals shared and unique transcriptional patterns across phage-host interactions.NAR genomics and bioinformatics · 2026Article
- END nucleases are antiphage defence systems targeting multiple phages with modified genomes.Nature microbiology · 2026Article
- Isolation and characterization of Enterococcus phages vB_EfaS_785CC and vB_EfaS_785CS.Functional & integrative genomics · 2026Article
- Phage isolation and functional characterization reveal strong antibiofilm activity againstFrontiers in cellular and infection microbiology · 2026Article
- Alterations of the Enteric Virome in Vogt-Koyanagi-Harada Disease.Investigative ophthalmology & visual science · 2025Article
- Characterization and genomic analysis of Sharanji: a jumbo bacteriophage of Escherichia coli.Virology journal · 2025Article
- Phage-phage competition and biofilms affect interactions between two virulent bacteriophages and Pseudomonas aeruginosa.The ISME journal · 2025Article
- Comparative transcriptomes reveal differential effects on host metabolism reprogramming in two different pelagiphage-SAR11 infection systems.ISME communications · 2025Article
- Nucleus-forming jumbophage PhiKZ therapeutically outcompetes non-nucleus-forming jumbophage Callisto.iScience · 2024Article
- Refining the transcriptional landscapes for distinct clades of virulent phages infectingmicroLife · 2024Article
- Obtaining Detailed Phage Transcriptomes Using ONT-Cappable-Seq.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Phage-Host Interaction Analysis Using Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Comparative genomics and proteomics analysis of phages infecting multi-drug resistant Escherichia coli O177 isolated from cattle faeces.Scientific reports · 2023Article
- Characterization and Comparative Genomic Analysis of Three VirulentInternational journal of molecular sciences · 2023Article
- Article
- Impact of phage predation onRNA biology · 2023Article
- Interactions between Jumbo Phage SA1 andMicroorganisms · 2022Article
- Global Transcriptomic Analysis of Bacteriophage-Host Interactions between a Kayvirus Therapeutic Phage and Staphylococcus aureus.Microbiology spectrum · 2022Article
- Genome-driven elucidation of phage-host interplay and impact of phage resistance evolution on bacterial fitness.The ISME journal · 2022Article
- Phage-Host Interaction Analysis by Flow Cytometry Allows for Rapid and Efficient Screening of Phages.Antibiotics (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
Pseudomonas virus vB_PaeM_PA5oct is proposed as a model jumbo bacteriophage to investigate phage-bacteria interactions and is a candidate for phage therapy applications. Combining hybrid sequencing, RNA-Seq and mass spectrometry allowed us to accurately annotate its 286,783 bp genome with 461 coding regions including four non-coding RNAs (ncRNAs) and 93 virion-associated proteins. PA5oct relies on the host RNA polymerase for the infection cycle and RNA-Seq revealed a gradual take-over of the total cell transcriptome from 21% in early infection to 93% in late infection. PA5oct is not organized into strictly contiguous regions of temporal transcription, but some genomic regions transcribed in early, middle and late phases of infection can be discriminated. Interestingly, we observe regions showing limited transcription activity throughout the infection cycle. We show that PA5oct upregulates specific bacterial operons during infection including operons pncA-pncB1-nadE involved in NAD biosynthesis, psl for exopolysaccharide biosynthesis and nap for periplasmic nitrate reductase production. We also observe a downregulation of T4P gene products suggesting mechanisms of superinfection exclusion. We used the proteome of PA5oct to position our isolate amongst other phages using a gene-sharing network. This integrative omics study illustrates the molecular diversity of jumbo viruses and raises new questions towards cellular regulation and phage-encoded hijacking mechanisms.
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Registered trials
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