ArticleJournal of clinical tuberculosis and other mycobacterial diseases2020
A scalable, efficient, and safe method to prepare high quality DNA from mycobacteria and other challenging cells.
Article in Journal of clinical tuberculosis and other mycobacterial diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- DNA sequencing for microbial surveillance in cystic fibrosis airways: advances, challenges, and clinical translation.Clinical microbiology reviews · 2026Review
- Novel Hawai'i and Pacific Island circulating clusters ofApplied and environmental microbiology · 2026Article
- Documenting Environmentally Acquired Mycobacterium intracellulare subsp. chimaera Pulmonary Disease Soon After Bronchiectasis Onset.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2026Article
- Evaluation of DNA extraction methods from clinical Mycobacterium tuberculosis primary liquid culture for whole-genome sequencing.BMC genomics · 2026Article
- Article
- A universal, high-quality, and high-yield DNA purification method for mycobacteria, includingMicrobiology spectrum · 2025Article
- Genomic and epidemiologic investigation of Mycobacterium abscessus isolates in a cystic fibrosis center to determine potential routes of transmission.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2025Article
- Consequences of Climate Change on the Emergence of Pathogenic, Environmentally Acquired Nontuberculous Mycobacteria.Open forum infectious diseases · 2025Article
- The Global Outbreak ofPathogens (Basel, Switzerland) · 2025Article
- First isolation of a methanotrophicApplied and environmental microbiology · 2025Article
- Culture and real-time quantitative PCR to detect environmental nontuberculous mycobacteria in a clinical care center.Total environment microbiology · 2025Article
- Genomic epidemiology ofERJ open research · 2024Article
- Common Features of Environmental Mycobacterium chelonae from Colorado Using Partial and Whole Genomic Sequence Analyses.Current microbiology · 2024Article
- Article
- Assessment and validation of enrichment and target capture approaches to improveJournal of clinical microbiology · 2023Article
- A quick, easy and efficient protocol for extracting high-quality RNA from Mycobacterium tuberculosis using a spin column commercial kit.BMC research notes · 2023Article
- Molecular Epidemiologic Investigation ofAnnals of the American Thoracic Society · 2023Article
- Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai'i.Frontiers in microbiology · 2023Article
- Clinical Evaluation of Metagenomic Next-Generation Sequencing and Identification of Risk Factors in Patients with Severe Community-Acquired Pneumonia.Infection and drug resistance · 2023Article
- Complete genome assembly of Hawai'i environmental nontuberculous mycobacteria reveals unexpected co-isolation with methylobacteria.PloS one · 2023Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid development in sequencing technology is creating an increase in demand for largely intact DNA as starting material as very long strands of DNA are sequenced directly to generate reads that are thousands of bases long. Organisms with thick cell walls are difficult to lyse, often impacting both DNA recovery and quality. Consequently, most mycobacterial DNA extraction methods require bead-beating steps or toxic chemicals. Here we present an updated method that yields abundant, high quality genomic DNA from
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Registered trials
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