ArticlePlant cell reports2020
CRISPR/Cas9-mediated mutagenesis of Clpsk1 in watermelon to confer resistance to Fusarium oxysporum f.sp. niveum.
Article in Plant cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Genome editing for vegetable crop improvement: Challenges and future prospects.Frontiers in genetics · 2022Pooled it
- Molecular Targets and Trait Innovation in CRISPR-Edited Vegetable Crops: An Up-to-Date Review (2020-2026).Plants (Basel, Switzerland) · 2026Review
- CRISPR-enabled functional genomics for bolstering plant tolerance to abiotic and biotic stress; a comprehensive review.Functional & integrative genomics · 2026Review
- Genomic resources and genetic improvement of vital tropical and subtropical fruit crops: current status and prospects.AoB PLANTS · 2025Review
- Turning Susceptibility into Strength: A New Era of Durable Resistance in Plants Through Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Genome editing in vegetable crops: a new era of sustainable agriculture.Molecular biology reports · 2025Review
- From Morphology to Multi-Omics: A New Age of Fusarium Research.Pathogens (Basel, Switzerland) · 2025Review
- Genetic and molecular approaches for Fusarium wilt resistance in garden pea: advances and future outlook.Plant molecular biology · 2025Review
- CRISPR mediated gene editing for economically important traits in horticultural crops: progress and prospects.Transgenic research · 2025Review
- Novel methods for genetic transformation of watermelon (Molecular breeding : new strategies in plant improvement · 2025Article
- Unlocking the potential of 'Egusi' melon (Frontiers in plant science · 2025Review
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- CRISPR/Cas-Mediated Genome Engineering in Plants: Application and Prospectives.Plants (Basel, Switzerland) · 2024Review
- Review
- Genome-Wide Identification and Expression Analysis of Chitinase Genes in Watermelon under Abiotic Stimuli andInternational journal of molecular sciences · 2024Article
- Sulfated peptides: key players in plant development, growth, and stress responses.Frontiers in plant science · 2024Review
- Ectopic expression of a truncated NLR gene from wildFrontiers in plant science · 2024Article
- CRISPR-Cas technology secures sustainability through its applications: a review in green biotechnology.3 Biotech · 2023Review
- Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis.Planta · 2023Review
- Applications of CRISPR/Cas genome editing in economically important fruit crops: recent advances and future directions.Molecular horticulture · 2023Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
key messageCRISPR/Cas9-mediated editing of Clpsk1 enhanced watermelon resistance to Fusarium oxysporum. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has proven to be an effective genome-editing tool for crop improvement. Previous studies described that Phytosulfokine (PSK) signalling attenuates plant immune response. In this work, we employed the CRISPR/Cas9 system to knockout Clpsk1 gene, encoding the PSK precursor, to confer enhanced watermelon resistance to Fusarium oxysporum f.sp. niveum (FON). Interactions between PSK and FON were analysed and it was found that transcript of Clpsk1 was significantly induced upon FON infection. Meanwhile, application of exogenous PSK increased the pathogen growth. Then, one sgRNA, which targeted the first exon of Clpsk1, was selected for construction of pRGEB32-CAS9-gRNA-Clpsk1 expression cassette. The construct was then transformed to watermelon through Agrobacterium tumefaciens-mediated transformation method. Six mutant plants were obtained and three types of mutations at the expected position were identified based on Sanger sequencing. Resistance evaluation indicated that Clpsk1 loss-of-function rendered watermelon seedlings more resistant to infection by FON. These results indicate that CRISPR/Cas9-mediated gene modification is an effective approach for watermelon improvement.
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Registered trials
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