ArticleScientific reports2020
Comparison of several Real-Time PCR Kits versus a Culture-dependent Algorithm to Identify Enteropathogens in Stool Samples.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Article
- Fifty Years of Non-pestis Yersinia Outbreaks in the United States: Patterns, Vehicles, and Surveillance Challenges, 1971-2024.Journal of food science · 2026Article
- Utility of Fecal Biomarkers to Predict Invasive Nature of Acute Infectious Diarrhea in Indian Children.Indian pediatrics · 2025Article
- Diagnosing gastrointestinal infections based on cycle threshold cut-offs of PCR.Microbiology spectrum · 2025Article
- Can Wastewater Surveillance Enhance Genomic Tracking of Climate-Driven Pathogens?Microorganisms · 2025Review
- Syndromic Gastrointestinal Panel Diagnostic Tests Have Changed our Understanding of the Epidemiology ofOpen forum infectious diseases · 2024Article
- Experience with PCR Testing for Enteric Bacteria and Viruses of Emergency Department Patients with Acute Gastroenteritis: Are There Implications for the Early Treatment ofAntibiotics (Basel, Switzerland) · 2024Article
- The effect of changing diagnostic method from culture to PCR on the number of episodes of human campylobacteriosis in Denmark: a retrospective study (2015-2022).Microbiology spectrum · 2024Article
- Rapid Reverse Purification DNA Extraction Approaches to Identify Microbial Pathogens in Wastewater.Microorganisms · 2023Article
- Detection of Foodborne Pathogens in Acute Gastroenteritis Patient's Stool Samples Using the BioFireMicroorganisms · 2022Article
- Molecular Identification ofAnimals : an open access journal from MDPI · 2022Article
- Nucleic Acid-Based Lateral Flow Biosensor forDiagnostics (Basel, Switzerland) · 2021Article
- Safety and Competency are the Main Priorities in Pediatric Endoscopy.Clinical endoscopy · 2020Article
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5 authors.
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Abstract
This study aims to validate the current diagnostic method for the clinical detection of gastroenteritis. We analyzed 400 stool samples to detect three of the most common enteropathogens: Salmonella spp., Campylobacter spp., and Yersinia enterocolitica. All specimens were tested with a routine clinical diagnosis algorithm and with five real-time PCR assays. A total of 98 specimens (24.5%) were positive for enteropathogens. We found 24 samples positive for Salmonella enterica, 71 positive for Campylobacter spp., and 4 positive for Yersinia enterocolitica. All evaluated methods exhibited a good performance in identifying Salmonella and Yersinia enterocolitica, being the highest positive percent agreement (PPA) value of 95.8% and 100%, respectively. The clinical algorithm showed the highest PPA value identifying Salmonella, due to the enrichment in selenite broth. However, the evaluated methods showed notable differences in the identification of Campylobacter species, obtaining a wide range of PPA values: 59.2%-100%. The clinical algorithm showed the lowest PPA value since it was only able to detect Campylobacter jejuni and Campylobacter coli species. This study revealed the importance of implementing the real-time PCR technique in a clinical algorithm: it improved the accuracy of the diagnosis and provided results in a shorter time compared to routine clinical methods.
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